7w7u
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The 'Ca2+-unbound' BeF3- of SERCA2b== |
| + | <StructureSection load='7w7u' size='340' side='right'caption='[[7w7u]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7w7u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W7U FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7u OCA], [https://pdbe.org/7w7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7u RCSB], [https://www.ebi.ac.uk/pdbsum/7w7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [https://www.uniprot.org/uniprot/AT2A2_HUMAN AT2A2_HUMAN] Darier disease;Acrokeratosis verruciformis of Hopf. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AT2A2_HUMAN AT2A2_HUMAN] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (By similarity).[UniProtKB:O55143]<ref>PMID:16402920</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sarco/endoplasmic reticulum Ca(2+) ATPase (SERCA) pumps Ca(2+) into the endoplasmic reticulum (ER). Herein, we present cryo-electron microscopy (EM) structures of three intermediates of SERCA2b: Ca(2+)-bound phosphorylated (E1P.2Ca(2+)) and Ca(2+)-unbound dephosphorylated (E2.Pi) intermediates and another between the E2P and E2.Pi states. Our cryo-EM analysis demonstrates that the E1P.2Ca(2+) state exists in low abundance and preferentially transitions to an E2P-like structure by releasing Ca(2+) and that the Ca(2+) release gate subsequently undergoes stepwise closure during the dephosphorylation processes. Importantly, each intermediate adopts multiple sub-state structures including those like the next one in the catalytic series, indicating conformational overlap at transition steps, as further substantiated by atomistic molecular dynamic simulations of SERCA2b in a lipid bilayer. The present findings provide insight into how enzymes accelerate catalytic cycles. | ||
| - | + | Multiple sub-state structures of SERCA2b reveal conformational overlap at transition steps during the catalytic cycle.,Zhang Y, Kobayashi C, Cai X, Watanabe S, Tsutsumi A, Kikkawa M, Sugita Y, Inaba K Cell Rep. 2022 Dec 6;41(10):111760. doi: 10.1016/j.celrep.2022.111760. PMID:36476867<ref>PMID:36476867</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7w7u" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Inaba K]] | ||
| + | [[Category: Tsutsumi A]] | ||
| + | [[Category: Watanabe S]] | ||
| + | [[Category: Zhang Y]] | ||
Current revision
The 'Ca2+-unbound' BeF3- of SERCA2b
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