This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1d2s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1d2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2s, resolution 1.55&Aring;" /> '''CRYSTAL STRUCTURE O...)
Current revision (15:45, 13 March 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1d2s.gif|left|200px]]<br />
 
-
<applet load="1d2s" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1d2s, resolution 1.55&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE==
-
Human sex hormone-binding globulin (SHBG) transports sex steroids in blood, and regulates their access to target tissues. In biological fluids, SHBG, exists as a homodimer and each monomer comprises two laminin G-like, domains (G domains). The crystal structure of the N-terminal G domain of, SHBG in complex with 5alpha-dihydrotestosterone at 1.55 A resolution, reveals both the architecture of the steroid-binding site and the, quaternary structure of the dimer. We also show that G domains have, jellyroll topology and are structurally related to pentraxin. In each SHBG, monomer, the steroid intercalates into a hydrophobic pocket within the, beta-sheet sandwich. The steroid and a 20 A distant calcium ion are not, located at the dimer interface. Instead, two separate steroid-binding, pockets and calcium-binding sites exist per dimer. The structure displays, intriguing disorder for loop segment Pro130-Arg135. In all other jellyroll, proteins, this loop is well ordered. If modelled accordingly, it covers, the steroid-binding site and could thereby regulate access of ligands to, the binding pocket.
+
<StructureSection load='1d2s' size='340' side='right'caption='[[1d2s]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1d2s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The August 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Anabolic Steroids'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_8 10.2210/rcsb_pdb/mom_2007_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D2S FirstGlance]. <br>
-
1D2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and DHT as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2S with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb92_1.html Anabolic Steroids]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D2S OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DHT:5-ALPHA-DIHYDROTESTOSTERONE'>DHT</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2s OCA], [https://pdbe.org/1d2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d2s RCSB], [https://www.ebi.ac.uk/pdbsum/1d2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2s ProSAT]</span></td></tr>
-
Crystal structure of human sex hormone-binding globulin: steroid transport by a laminin G-like domain., Grishkovskaya I, Avvakumov GV, Sklenar G, Dales D, Hammond GL, Muller YA, EMBO J. 2000 Feb 15;19(4):504-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10675319 10675319]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SHBG_HUMAN SHBG_HUMAN] Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/1d2s_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d2s ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Anabolic Steroids]]
[[Category: Anabolic Steroids]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Avvakumov, G.V.]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Dales, D.]]
+
[[Category: Avvakumov GV]]
-
[[Category: Grishkovskaya, I.]]
+
[[Category: Dales D]]
-
[[Category: Hammond, G.L.]]
+
[[Category: Grishkovskaya I]]
-
[[Category: Muller, Y.A.]]
+
[[Category: Hammond GL]]
-
[[Category: Sklenar, G.]]
+
[[Category: Muller YA]]
-
[[Category: CA]]
+
[[Category: Sklenar G]]
-
[[Category: DHT]]
+
-
[[Category: androgen binding protein (abp)]]
+
-
[[Category: jellyroll]]
+
-
[[Category: laminin g-like domain]]
+
-
[[Category: sex steroid binding protein (sbp)]]
+
-
[[Category: steroid transport]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:27:58 2007''
+

Current revision

CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE

PDB ID 1d2s

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1d2s"
Personal tools