7e3z
From Proteopedia
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==Non-Ribosomal Peptide Synthetases, Thioesterase== | ==Non-Ribosomal Peptide Synthetases, Thioesterase== | ||
| - | <StructureSection load='7e3z' size='340' side='right'caption='[[7e3z]]' scene=''> | + | <StructureSection load='7e3z' size='340' side='right'caption='[[7e3z]], [[Resolution|resolution]] 1.45Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3Z FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E3Z FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3z OCA], [https://pdbe.org/7e3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3z RCSB], [https://www.ebi.ac.uk/pdbsum/7e3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3z ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e3z OCA], [https://pdbe.org/7e3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e3z RCSB], [https://www.ebi.ac.uk/pdbsum/7e3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e3z ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Systematic inactivation of nonribosomal peptide synthetase (NRPS) domains and translocation of the thioesterase (TE) domain revealed several unprecedented nonlinear NRPS assembly processes during the biosynthesis of the cyclodepsipeptide WS9326A in Streptomyces sp. SNM55. First, two sets of type IotaIota TE (TEIotaIota)-like enzymes mediate the shuttling of activated amino acids between two sets of stand-alone adenylation (A)-thiolation (T) didomain modules and an "A-less" condensation (C)-T module with distinctive specificities and flexibilities. This was confirmed by the elucidation of the affinities of the A-T didomains for the TEIotaIotas and its structure. Second, the C-T didomain module operates iteratively and independently from other modules in the same protein to catalyze two chain elongation cycles. Third, this biosynthetic pathway includes the first example of module skipping, where the interpolated C and T domains are required for chain transfer. | ||
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| + | Unprecedented Noncanonical Features of the Nonlinear Nonribosomal Peptide Synthetase Assembly Line for WS9326A Biosynthesis.,Kim MS, Bae M, Jung YE, Kim JM, Hwang S, Song MC, Ban YH, Bae ES, Hong S, Lee SK, Cha SS, Oh DC, Yoon YJ Angew Chem Int Ed Engl. 2021 Sep 1;60(36):19766-19773. doi:, 10.1002/anie.202103872. Epub 2021 Jun 17. PMID:33963654<ref>PMID:33963654</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e3z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Non-Ribosomal Peptide Synthetases, Thioesterase
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