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| - | [[Image:1fzp.gif|left|200px]]
| + | REMOVED: The PDB entry 1fzp was removed. |
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| - | {{STRUCTURE_1fzp| PDB=1fzp | SCENE= }}
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| - | '''CRYSTAL STRUCTURES OF SARA: A PLEIOTROPIC REGULATOR OF VIRULENCE GENES IN S. AUREUS'''
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| - | ==Overview==
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| - | Staphylococcus aureus is a major human pathogen, the potency of which can be attributed to the regulated expression of an impressive array of virulence determinants. A key pleiotropic transcriptional regulator of these virulence factors is SarA, which is encoded by the sar (staphylococcal accessory regulator) locus. SarA was characterized initially as an activator of a second virulence regulatory locus, agr, through its interaction with a series of heptad repeats (AGTTAAG) within the agr promoter. Subsequent DNA-binding studies have revealed that SarA binds readily to multiple AT-rich sequences of variable lengths. Here we describe the crystal structure of SarA and a SarA-DNA complex at resolutions of 2.50 A and 2.95 A, respectively. SarA has a fold consisting of a four-helix core region and 'inducible regions' comprising a beta-hairpin and a carboxy-terminal loop. On binding DNA, the inducible regions undergo marked conformational changes, becoming part of extended and distorted alpha-helices, which encase the DNA. SarA recognizes an AT-rich site in which the DNA is highly overwound and adopts a D-DNA-like conformation by indirect readout. These structures thus provide insight into SarA-mediated transcription regulation.
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| - | ==About this Structure==
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| - | 1FZP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZP OCA].
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| - | ==Reference==
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| - | Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus., Schumacher MA, Hurlburt BK, Brennan RG, Nature. 2001 Jan 11;409(6817):215-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11196648 11196648]
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| - | [[Category: Single protein]]
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| - | [[Category: Staphylococcus aureus]]
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| - | [[Category: Brennan, R G.]]
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| - | [[Category: Hurlburt, B.]]
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| - | [[Category: Schumacher, M A.]]
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| - | [[Category: Conformational changes,coil to helix]]
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| - | [[Category: D-dna]]
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| - | [[Category: Indirect readout]]
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| - | [[Category: Protein-dna complex]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:56:53 2008''
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