This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3a55

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of the A47Q2 mutant of pro- protein-glutaminase

Structural highlights

3a55 is a 2 chain structure with sequence from Chryseobacterium proteolyticum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9AQQ8_9FLAO

Publication Abstract from PubMed

Protein glutaminase, which converts a protein glutamine residue to a glutamate residue, is expected to be useful as a new food-processing enzyme. The crystal structures of the mature and pro forms of the enzyme were refined at 1.15 and 1.73 A resolution, respectively. The overall structure of the mature enzyme has a weak homology to the core domain of human transglutaminase-2. The catalytic triad (Cys-His-Asp) common to transglutaminases and cysteine proteases is located in the bottom of the active site pocket. The structure of the recombinant pro form shows that a short loop between S2 and S3 in the proregion covers and interacts with the active site of the mature region, mimicking the protein substrate of the enzyme. Ala-47 is located just above the pocket of the active site. Two mutant structures (A47Q-1 and A47Q-2) refined at 1.5 A resolution were found to correspond to the enzyme-substrate complex and an S-acyl intermediate. Based on these structures, the catalytic mechanism of protein glutaminase is proposed.

Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex.,Hashizume R, Maki Y, Mizutani K, Takahashi N, Matsubara H, Sugita A, Sato K, Yamaguchi S, Mikami B J Biol Chem. 2011 Nov 4;286(44):38691-702. Epub 2011 Sep 16. PMID:21926168^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hashizume R, Maki Y, Mizutani K, Takahashi N, Matsubara H, Sugita A, Sato K, Yamaguchi S, Mikami B. Crystal structures of protein glutaminase and its pro forms converted into enzyme-substrate complex. J Biol Chem. 2011 Nov 4;286(44):38691-702. Epub 2011 Sep 16. PMID:21926168 doi:10.1074/jbc.M111.255133

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3a55"

Categories: Chryseobacterium proteolyticum | Large Structures | Hashizume R | Mikami B | Yamaguchi S

@@ Line 3: / Line 3: @@
 <StructureSection load='3a55' size='340' side='right'caption='[[3a55]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3a55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"chryseobacterium_proteolyticum"_yamaguchi_and_yokoe_2000 "chryseobacterium proteolyticum" yamaguchi and yokoe 2000]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A55 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3a55]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chryseobacterium_proteolyticum Chryseobacterium proteolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A55 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2zk9|2zk9]], [[3a54|3a54]], [[3a56|3a56]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=118127 "Chryseobacterium proteolyticum" Yamaguchi and Yokoe 2000])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a55 OCA], [https://pdbe.org/3a55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a55 RCSB], [https://www.ebi.ac.uk/pdbsum/3a55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a55 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9AQQ8_9FLAO Q9AQQ8_9FLAO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Chryseobacterium proteolyticum yamaguchi and yokoe 2000]]
+[[Category: Chryseobacterium proteolyticum]]
 [[Category: Large Structures]]
-[[Category: Hashizume, R]]
+[[Category: Hashizume R]]
-[[Category: Mikami, B]]
+[[Category: Mikami B]]
-[[Category: Yamaguchi, S]]
+[[Category: Yamaguchi S]]
-[[Category: Hydrolase]]
-[[Category: Mutant structure like the reaction intermediate]]

3a55

From Proteopedia

Current revision

Crystal structure of the A47Q2 mutant of pro- protein-glutaminase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox