1g01

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[[Image:1g01.gif|left|200px]]
 
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==ALKALINE CELLULASE K CATALYTIC DOMAIN==
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The line below this paragraph, containing "STRUCTURE_1g01", creates the "Structure Box" on the page.
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<StructureSection load='1g01' size='340' side='right'caption='[[1g01]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1g01]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._KSM-635 Bacillus sp. KSM-635]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G01 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_1g01| PDB=1g01 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g01 OCA], [https://pdbe.org/1g01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g01 RCSB], [https://www.ebi.ac.uk/pdbsum/1g01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g01 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUN_BACS6 GUN_BACS6]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g0/1g01_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g01 ConSurf].
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<div style="clear:both"></div>
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'''ALKALINE CELLULASE K CATALYTIC DOMAIN'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of the catalytic domain of alkaline cellulase K was determined at 1.9 A resolution. Because of the most alkaliphilic nature and it's highest activity at pH 9.5, it is used commercially in laundry detergents. An analysis of the structural bases of the alkaliphilic character of the enzyme suggested a mechanism similar to that previously proposed for alkaline proteases, that is, an increase in the number of Arg, His, and Gln residues, and a decrease in Asp and Lys residues. Some ion pairs were formed by the gained Arg residues, which is similar to what has been found in the alkaline proteases. Lys-Asp ion pairs are disfavored and partly replaced with Arg-Asp ion pairs. The alkaline adaptation appeared to be a remodeling of ion pairs so that the charge balance is kept in the high pH range.
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[[Category: Bacillus sp. KSM-635]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Hakamada Y]]
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1G01 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G01 OCA].
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[[Category: Ishida H]]
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[[Category: Ito S]]
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==Reference==
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[[Category: Noda J]]
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Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme., Shirai T, Ishida H, Noda J, Yamane T, Ozaki K, Hakamada Y, Ito S, J Mol Biol. 2001 Jul 27;310(5):1079-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11501997 11501997]
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[[Category: Ozaki K]]
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[[Category: Bacillus sp.]]
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[[Category: Shirai T]]
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[[Category: Cellulase]]
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[[Category: Yamane T]]
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[[Category: Single protein]]
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[[Category: Hakamada, Y.]]
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[[Category: Ishida, H.]]
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[[Category: Ito, S.]]
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[[Category: Noda, J.]]
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[[Category: Ozaki, K.]]
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[[Category: Shirai, T.]]
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[[Category: Yamane, T.]]
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[[Category: Alpha/beta barrel]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:57:37 2008''
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Current revision

ALKALINE CELLULASE K CATALYTIC DOMAIN

PDB ID 1g01

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