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| | <StructureSection load='6t6m' size='340' side='right'caption='[[6t6m]], [[Resolution|resolution]] 1.49Å' scene=''> | | <StructureSection load='6t6m' size='340' side='right'caption='[[6t6m]], [[Resolution|resolution]] 1.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6t6m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aphanocapsa_sp._(strain_n-1) Aphanocapsa sp. (strain n-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T6M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6t6m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6T6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6T6M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6t6k|6t6k]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slr1963 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1148 Aphanocapsa sp. (strain N-1)])</td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t6m OCA], [https://pdbe.org/6t6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t6m RCSB], [https://www.ebi.ac.uk/pdbsum/6t6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t6m ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6t6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6t6m OCA], [https://pdbe.org/6t6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6t6m RCSB], [https://www.ebi.ac.uk/pdbsum/6t6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6t6m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3]] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref>
| + | [https://www.uniprot.org/uniprot/OCP_SYNY3 OCP_SYNY3] Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.<ref>PMID:16531492</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Botnarevskiy, V S]] | + | [[Category: Synechocystis sp. PCC 6803]] |
| - | [[Category: Gordeliy, V]] | + | [[Category: Botnarevskiy VS]] |
| - | [[Category: Gushchin, I]] | + | [[Category: Gordeliy V]] |
| - | [[Category: Kovalev, K]] | + | [[Category: Gushchin I]] |
| - | [[Category: Maksimov, E G]] | + | [[Category: Kovalev K]] |
| - | [[Category: Remeeva, A]] | + | [[Category: Maksimov EG]] |
| - | [[Category: Slonimskiy, Y B]] | + | [[Category: Remeeva A]] |
| - | [[Category: Sluchanko, N N]] | + | [[Category: Slonimskiy YB]] |
| - | [[Category: Stepanov, A V]] | + | [[Category: Sluchanko NN]] |
| - | [[Category: Mutant]]
| + | [[Category: Stepanov AV]] |
| - | [[Category: Orange carotenoid protein]]
| + | |
| - | [[Category: Photoactive protein]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| Structural highlights
Function
OCP_SYNY3 Acts as a photo-protectant. Essential for inhibiting white and blue-green light non-photochemical quenching (NPQ). Binding carotenoids improves OCP's intrinsic photoprotectant activity by broadening its absorption spectrum and facilitating the dissipation of absorbed energy.[1]
Publication Abstract from PubMed
Here, we propose a possible photoactivation mechanism of a 35-kDa blue light-triggered photoreceptor, the Orange Carotenoid Protein (OCP), suggesting that the reaction involves the transient formation of a protonated ketocarotenoid (oxocarbenium cation) state. Taking advantage of engineering an OCP variant carrying the Y201W mutation, which shows superior spectroscopic and structural properties, it is shown that the presence of Trp201 augments the impact of one critical H-bond between the ketocarotenoid and the protein. This confers an unprecedented homogeneity of the dark-adapted OCP state and substantially increases the yield of the excited photoproduct S*, which is important for the productive photocycle to proceed. A 1.37 A crystal structure of OCP Y201W combined with femtosecond time-resolved absorption spectroscopy, kinetic analysis, and deconvolution of the spectral intermediates, as well as extensive quantum chemical calculations incorporating the effect of the local electric field, highlighted the role of charge-transfer states during OCP photoconversion.
Role of hydrogen bond alternation and charge transfer states in photoactivation of the Orange Carotenoid Protein.,Yaroshevich IA, Maksimov EG, Sluchanko NN, Zlenko DV, Stepanov AV, Slutskaya EA, Slonimskiy YB, Botnarevskii VS, Remeeva A, Gushchin I, Kovalev K, Gordeliy VI, Shelaev IV, Gostev FE, Khakhulin D, Poddubnyy VV, Gostev TS, Cherepanov DA, Polivka T, Kloz M, Friedrich T, Paschenko VZ, Nadtochenko VA, Rubin AB, Kirpichnikov MP Commun Biol. 2021 May 10;4(1):539. doi: 10.1038/s42003-021-02022-3. PMID:33972665[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wilson A, Ajlani G, Verbavatz JM, Vass I, Kerfeld CA, Kirilovsky D. A soluble carotenoid protein involved in phycobilisome-related energy dissipation in cyanobacteria. Plant Cell. 2006 Apr;18(4):992-1007. Epub 2006 Mar 10. PMID:16531492 doi:10.1105/tpc.105.040121
- ↑ Yaroshevich IA, Maksimov EG, Sluchanko NN, Zlenko DV, Stepanov AV, Slutskaya EA, Slonimskiy YB, Botnarevskii VS, Remeeva A, Gushchin I, Kovalev K, Gordeliy VI, Shelaev IV, Gostev FE, Khakhulin D, Poddubnyy VV, Gostev TS, Cherepanov DA, Polivka T, Kloz M, Friedrich T, Paschenko VZ, Nadtochenko VA, Rubin AB, Kirpichnikov MP. Role of hydrogen bond alternation and charge transfer states in photoactivation of the Orange Carotenoid Protein. Commun Biol. 2021 May 10;4(1):539. doi: 10.1038/s42003-021-02022-3. PMID:33972665 doi:http://dx.doi.org/10.1038/s42003-021-02022-3
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