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3b0f

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<StructureSection load='3b0f' size='340' side='right'caption='[[3b0f]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='3b0f' size='340' side='right'caption='[[3b0f]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3b0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B0F FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3b0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B0F FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rru|2rru]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sqstm1, A170, STAP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b0f OCA], [https://pdbe.org/3b0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b0f RCSB], [https://www.ebi.ac.uk/pdbsum/3b0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b0f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b0f OCA], [https://pdbe.org/3b0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b0f RCSB], [https://www.ebi.ac.uk/pdbsum/3b0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b0f ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/SQSTM_MOUSE SQSTM_MOUSE]] Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD.<ref>PMID:14960283</ref> <ref>PMID:18382763</ref>
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[https://www.uniprot.org/uniprot/SQSTM_MOUSE SQSTM_MOUSE] Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD.<ref>PMID:14960283</ref> <ref>PMID:18382763</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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p62/SQSTM1/A170 is a multimodular protein that is found in ubiquitin-positive inclusions associated with neurodegenerative diseases. Recent findings indicate that p62 mediates the interaction between ubiquitinated proteins and autophagosomes, leading these proteins to be degraded via the autophagy-lysosomal pathway. This ubiquitin-mediated selective autophagy is thought to begin with recognition of the ubiquitinated proteins by the C-terminal ubiquitin-associated (UBA) domain of p62. We present here the crystal structure of the UBA domain of mouse p62 and the solution structure of its ubiquitin-bound form. The p62 UBA domain adopts a novel dimeric structure in crystals, which is distinctive from those of other UBA domains. NMR analyses reveal that in solution the domain exists in equilibrium between the dimer and monomer forms, and binding ubiquitin shifts the equilibrium toward the monomer to form a 1:1 complex between the UBA domain and ubiquitin. The dimer-to-monomer transition is associated with a structural change of the very C-terminal end of the p62 UBA domain, although the UBA fold itself is essentially maintained. Our data illustrate that dimerization and ubiquitin binding of the p62 UBA domain are incompatible with each other. These observations reveal an autoinhibitory mechanism in the p62 UBA domain and suggest that autoinhibition plays a role in the function of p62.
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Crystal structure of the ubiquitin-associated (UBA) domain of p62 and its interaction with ubiquitin.,Isogai S, Morimoto D, Arita K, Unzai S, Tenno T, Hasegawa J, Sou YS, Komatsu M, Tanaka K, Shirakawa M, Tochio H J Biol Chem. 2011 Sep 9;286(36):31864-74. Epub 2011 Jun 29. PMID:21715324<ref>PMID:21715324</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3b0f" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Mus musculus]]
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[[Category: Arita, K]]
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[[Category: Arita K]]
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[[Category: Hasegawa, J]]
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[[Category: Hasegawa J]]
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[[Category: Isogai, S]]
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[[Category: Isogai S]]
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[[Category: Komatsu, M]]
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[[Category: Komatsu M]]
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[[Category: Morimoto, D]]
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[[Category: Morimoto D]]
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[[Category: Shirakawa, M]]
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[[Category: Shirakawa M]]
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[[Category: Sou, Y]]
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[[Category: Sou Y]]
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[[Category: Tanaka, K]]
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[[Category: Tanaka K]]
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[[Category: Tenno, T]]
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[[Category: Tenno T]]
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[[Category: Tochio, H]]
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[[Category: Tochio H]]
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[[Category: Unzai, S]]
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[[Category: Unzai S]]
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[[Category: Autophagy]]
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[[Category: Protein binding]]
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[[Category: Ubiquitin]]
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Current revision

Crystal structure of the UBA domain of p62 and its interaction with ubiquitin

PDB ID 3b0f

Drag the structure with the mouse to rotate

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