1g3i

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[[Image:1g3i.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE HSLUV PROTEASE-CHAPERONE COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1g3i", creates the "Structure Box" on the page.
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<StructureSection load='1g3i' size='340' side='right'caption='[[1g3i]], [[Resolution|resolution]] 3.41&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1g3i]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. The August 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''AAA+ Proteases'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_8 10.2210/rcsb_pdb/mom_2006_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G3I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.41&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
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{{STRUCTURE_1g3i| PDB=1g3i | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g3i OCA], [https://pdbe.org/1g3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g3i RCSB], [https://www.ebi.ac.uk/pdbsum/1g3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g3i ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF THE HSLUV PROTEASE-CHAPERONE COMPLEX'''
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== Function ==
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[https://www.uniprot.org/uniprot/HSLU_HAEIN HSLU_HAEIN] ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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HslUV is a "prokaryotic proteasome" composed of the HslV protease and the HslU ATPase, a chaperone of the Clp/Hsp100 family. The 3.4 A crystal structure of an HslUV complex is presented here. Two hexameric ATP binding rings of HslU bind intimately to opposite sides of the HslV protease; the HslU "intermediate domains" extend outward from the complex. The solution structure of HslUV, derived from small angle X-ray scattering data under conditions where the complex is assembled and active, agrees with this crystallographic structure. When the complex forms, the carboxy-terminal helices of HslU distend and bind between subunits of HslV, and the apical helices of HslV shift substantially, transmitting a conformational change to the active site region of the protease.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/1g3i_consurf.spt"</scriptWhenChecked>
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1G3I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. The following page contains interesting information on the relation of 1G3I with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3I OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal and solution structures of an HslUV protease-chaperone complex., Sousa MC, Trame CB, Tsuruta H, Wilbanks SM, Reddy VS, McKay DB, Cell. 2000 Nov 10;103(4):633-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11106733 11106733]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g3i ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: AAA+ Proteases]]
[[Category: AAA+ Proteases]]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: McKay, D B.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Reddy, V S.]]
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[[Category: McKay DB]]
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[[Category: Sousa, M C.]]
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[[Category: Reddy VS]]
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[[Category: Trame, C B.]]
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[[Category: Sousa MC]]
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[[Category: Tsuruta, H.]]
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[[Category: Trame CB]]
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[[Category: Wilbanks, S M.]]
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[[Category: Tsuruta H]]
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[[Category: Chaperone/hydrolase]]
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[[Category: Wilbanks SM]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:05:34 2008''
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CRYSTAL STRUCTURE OF THE HSLUV PROTEASE-CHAPERONE COMPLEX

PDB ID 1g3i

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