2j9i

From Proteopedia

(Difference between revisions)

Current revision

Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.

2j9i, resolution 17.00Å

Structural highlights

2j9i is a 12 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 17Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LGSN_MOUSE May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.^[1]

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lengsin is a major protein of the vertebrate eye lens. It belongs to the hitherto purely prokaryotic GS I branch of the glutamine synthetase (GS) superfamily, but has no enzyme activity. Like the taxon-specific crystallins, Lengsin is the result of the recruitment of an ancient enzyme to a noncatalytic role in the vertebrate lens. Cryo-EM and modeling studies of Lengsin show a dodecamer structure with important similarities and differences with prokaryotic GS I structures. GS homology regions of Lengsin are well conserved, but the N-terminal domain shows evidence of dynamic evolutionary changes. Compared with birds and fish, most mammals have an additional exon corresponding to part of the N-terminal domain; however, in human, this is a nonfunctional pseudoexon. Genes related to Lengsin are also present in the sea urchin, suggesting that this branch of the GS I family, supplanted by GS II enzymes in vertebrates, has an ancient role in metazoans.

Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens.,Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G Structure. 2006 Dec;14(12):1823-34. PMID:17161372^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wyatt K, Gao C, Tsai JY, Fariss RN, Ray S, Wistow G. A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lens. J Biol Chem. 2008 Mar 7;283(10):6607-15. PMID:18178558 doi:10.1074/jbc.M709144200
↑ Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G. Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens. Structure. 2006 Dec;14(12):1823-34. PMID:17161372 doi:http://dx.doi.org/10.1016/j.str.2006.10.008

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j9i"

@@ Line 3: / Line 3: @@
 <SX load='2j9i' size='340' side='right' viewer='molstar' caption='[[2j9i]], [[Resolution|resolution]] 17.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2j9i]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2j9i]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9I FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9i OCA], [https://pdbe.org/2j9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9i RCSB], [https://www.ebi.ac.uk/pdbsum/2j9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9i ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 17&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9i OCA], [https://pdbe.org/2j9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9i RCSB], [https://www.ebi.ac.uk/pdbsum/2j9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9i ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LGSN_MOUSE LGSN_MOUSE] May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.<ref>PMID:18178558</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 34: / Line 37: @@
 </SX>
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Bateman, O A]]
+[[Category: Bateman OA]]
-[[Category: Orlova, E V]]
+[[Category: Orlova EV]]
-[[Category: Slingsby, C]]
+[[Category: Slingsby C]]
-[[Category: Wang, L]]
+[[Category: Wang L]]
-[[Category: White, H E]]
+[[Category: White HE]]
-[[Category: Wistow, G]]
+[[Category: Wistow G]]
-[[Category: Wyatt, K]]
+[[Category: Wyatt K]]
-[[Category: Ligase]]

2j9i

From Proteopedia

Current revision

Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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