1g6v

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[[Image:1g6v.jpg|left|200px]]
 
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==Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase==
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The line below this paragraph, containing "STRUCTURE_1g6v", creates the "Structure Box" on the page.
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<StructureSection load='1g6v' size='340' side='right'caption='[[1g6v]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1g6v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6V FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1g6v| PDB=1g6v | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6v OCA], [https://pdbe.org/1g6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6v RCSB], [https://www.ebi.ac.uk/pdbsum/1g6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAH2_BOVIN CAH2_BOVIN] Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g6v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Detailed knowledge on antibody-antigen recognition is scarce given the unlimited antibody specificities of which only few have been investigated at an atomic level. We report the crystal structures of an antibody fragment derived from a camel heavy chain antibody against carbonic anhydrase, free and in complex with antigen. Surprisingly, this single-domain antibody interacts with nanomolar affinity with the antigen through its third hypervariable loop (19 amino acids long), providing a flat interacting surface of 620 A(2). For the first time, a single-domain antibody is observed with its first hypervariable loop adopting a type-1 canonical structure. The second hypervariable loop, of unique size due to a somatic mutation, reveals a regular beta-turn. The third hypervariable loop covers the remaining hypervariable loops and the side of the domain that normally interacts with the variable domain of the light chain. Specific amino acid substitutions and reoriented side chains reshape this side of the domain and increase its hydrophilicity. Of interest is the substitution of the conserved Trp-103 by Arg because it opens new perspectives to 'humanize' a camel variable domain of heavy chain of heavy chain antibody (VHH) or to 'camelize' a human or a mouse variable domain of heavy chain of conventional antibody (VH).
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'''Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase'''
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Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody.,Desmyter A, Decanniere K, Muyldermans S, Wyns L J Biol Chem. 2001 Jul 13;276(28):26285-90. Epub 2001 May 7. PMID:11342547<ref>PMID:11342547</ref>
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==Overview==
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Detailed knowledge on antibody-antigen recognition is scarce given the unlimited antibody specificities of which only few have been investigated at an atomic level. We report the crystal structures of an antibody fragment derived from a camel heavy chain antibody against carbonic anhydrase, free and in complex with antigen. Surprisingly, this single-domain antibody interacts with nanomolar affinity with the antigen through its third hypervariable loop (19 amino acids long), providing a flat interacting surface of 620 A(2). For the first time, a single-domain antibody is observed with its first hypervariable loop adopting a type-1 canonical structure. The second hypervariable loop, of unique size due to a somatic mutation, reveals a regular beta-turn. The third hypervariable loop covers the remaining hypervariable loops and the side of the domain that normally interacts with the variable domain of the light chain. Specific amino acid substitutions and reoriented side chains reshape this side of the domain and increase its hydrophilicity. Of interest is the substitution of the conserved Trp-103 by Arg because it opens new perspectives to 'humanize' a camel variable domain of heavy chain of heavy chain antibody (VHH) or to 'camelize' a human or a mouse variable domain of heavy chain of conventional antibody (VH).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1G6V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6V OCA].
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</div>
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<div class="pdbe-citations 1g6v" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody., Desmyter A, Decanniere K, Muyldermans S, Wyns L, J Biol Chem. 2001 Jul 13;276(28):26285-90. Epub 2001 May 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11342547 11342547]
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Camelus dromedarius]]
[[Category: Camelus dromedarius]]
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[[Category: Carbonate dehydratase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Decanniere K]]
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[[Category: Decanniere, K.]]
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[[Category: Desmyter A]]
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[[Category: Desmyter, A.]]
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[[Category: Muyldermans S]]
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[[Category: Muyldermans, S.]]
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[[Category: Wyns L]]
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[[Category: Wyns, L.]]
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[[Category: Antibody]]
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[[Category: Antigen]]
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[[Category: Complex]]
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[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:12:59 2008''
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Current revision

Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase

PDB ID 1g6v

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