1g70

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[[Image:1g70.gif|left|200px]]
 
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==COMPLEX OF HIV-1 RRE-IIB RNA WITH RSG-1.2 PEPTIDE==
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The line below this paragraph, containing "STRUCTURE_1g70", creates the "Structure Box" on the page.
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<StructureSection load='1g70' size='340' side='right'caption='[[1g70]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1g70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G70 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g70 OCA], [https://pdbe.org/1g70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g70 RCSB], [https://www.ebi.ac.uk/pdbsum/1g70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g70 ProSAT]</span></td></tr>
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{{STRUCTURE_1g70| PDB=1g70 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''COMPLEX OF HIV-1 RRE-IIB RNA WITH RSG-1.2 PEPTIDE'''
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== Publication Abstract from PubMed ==
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==Overview==
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We have used NMR spectroscopy to determine the solution structure of a complex between an oligonucleotide derived from stem IIB of the Rev responsive element (RRE-IIB) of HIV-1 mRNA and an in vivo selected, high affinity binding Arg-rich peptide. The peptide binds in a partially alpha-helical conformation into a pocket within the RNA deep groove. Comparison with the structure of a complex between an alpha-helical Rev peptide and RRE-IIB reveals that the sequence of the bound peptide determines the local conformation of the RRE peptide binding site. A conformational switch of an unpaired uridine base was revealed; this points out into the solvent in the Rev peptide complex, but it is stabilized inside the RNA deep groove by stacking with an Arg side chain in the selected peptide complex. The conformational switch has been visualized by NMR chemical shift mapping of the uridine H5/H6 atoms during a competition experiment in which Rev peptide was displaced from RRE-IIB by the higher affinity binding selected peptide.
We have used NMR spectroscopy to determine the solution structure of a complex between an oligonucleotide derived from stem IIB of the Rev responsive element (RRE-IIB) of HIV-1 mRNA and an in vivo selected, high affinity binding Arg-rich peptide. The peptide binds in a partially alpha-helical conformation into a pocket within the RNA deep groove. Comparison with the structure of a complex between an alpha-helical Rev peptide and RRE-IIB reveals that the sequence of the bound peptide determines the local conformation of the RRE peptide binding site. A conformational switch of an unpaired uridine base was revealed; this points out into the solvent in the Rev peptide complex, but it is stabilized inside the RNA deep groove by stacking with an Arg side chain in the selected peptide complex. The conformational switch has been visualized by NMR chemical shift mapping of the uridine H5/H6 atoms during a competition experiment in which Rev peptide was displaced from RRE-IIB by the higher affinity binding selected peptide.
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==About this Structure==
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Peptide-triggered conformational switch in HIV-1 RRE RNA complexes.,Gosser Y, Hermann T, Majumdar A, Hu W, Frederick R, Jiang F, Xu W, Patel DJ Nat Struct Biol. 2001 Feb;8(2):146-50. PMID:11175904<ref>PMID:11175904</ref>
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1G70 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G70 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Peptide-triggered conformational switch in HIV-1 RRE RNA complexes., Gosser Y, Hermann T, Majumdar A, Hu W, Frederick R, Jiang F, Xu W, Patel DJ, Nat Struct Biol. 2001 Feb;8(2):146-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11175904 11175904]
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</div>
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<div class="pdbe-citations 1g70" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Human immunodeficiency virus 1]]
[[Category: Human immunodeficiency virus 1]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Frederick, R.]]
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[[Category: Frederick R]]
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[[Category: Gosser, Y.]]
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[[Category: Gosser Y]]
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[[Category: Hermann, T.]]
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[[Category: Hermann T]]
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[[Category: Hu, W.]]
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[[Category: Hu W]]
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[[Category: Jiang, F.]]
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[[Category: Jiang F]]
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[[Category: Majumdar, A.]]
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[[Category: Majumdar A]]
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[[Category: Patel, D J.]]
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[[Category: Patel DJ]]
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[[Category: Xu, W.]]
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[[Category: Xu W]]
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[[Category: Non-canonical base pair]]
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[[Category: Peptide-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:13:18 2008''
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Current revision

COMPLEX OF HIV-1 RRE-IIB RNA WITH RSG-1.2 PEPTIDE

PDB ID 1g70

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