3cnc
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3cnc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_16 Human adenovirus 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CNC FirstGlance]. <br> | <table><tr><td colspan='2'>[[3cnc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_16 Human adenovirus 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CNC FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnc OCA], [https://pdbe.org/3cnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cnc RCSB], [https://www.ebi.ac.uk/pdbsum/3cnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnc ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnc OCA], [https://pdbe.org/3cnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cnc RCSB], [https://www.ebi.ac.uk/pdbsum/3cnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q67711_ADE16 Q67711_ADE16] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cnc ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cnc ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | A majority of species B adenoviruses (Ads) use CD46 as their primary receptor; however, the precise mechanisms involved in the binding of different Ad types to CD46 have not been resolved. Although previous studies indicate close similarities between two members of species B2 Ads in their usage of CD46, our current investigations revealed a surprisingly low CD46 binding affinity of the species B1 Ad16 fiber knob (equilibrium dissociation constant of 437 nM). We determined the crystal structure of the Ad16 fiber knob and constructed a model of this protein in complex with CD46. A comparison of this model to that of the CD46-Ad11 complex revealed structural differences in the FG and IJ loops that are part of the CD46 binding site. An analysis of a panel of recombinant fiber knobs with mutations targeting these regions in Ad16 and Ad11 uncovered a major contribution of the FG loop on CD46 binding. Two extra residues in the FG loop of the Ad16 fiber significantly reduce receptor interaction. Although avidity effects permit the use of CD46 on host cells by Ad16, virus binding occurs with lower efficiency than with B2 Ad types. The longer FG loop of the Ad16 fiber knob also is shared by other species B1 Ad fibers and, thus, may contribute to the low CD46 binding efficiencies observed for these Ad types. Our findings provide a better understanding of how different Ad types associate with CD46 and could aid in the selection of specific Ad fibers for more efficient Ad gene delivery vectors. | ||
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| - | Structural variations in species B adenovirus fibers impact CD46 association.,Pache L, Venkataraman S, Reddy VS, Nemerow GR J Virol. 2008 Aug;82(16):7923-31. Epub 2008 Jun 4. PMID:18524830<ref>PMID:18524830</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3cnc" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Human adenovirus 16]] | [[Category: Human adenovirus 16]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Nemerow | + | [[Category: Nemerow GR]] |
| - | [[Category: Pache | + | [[Category: Pache L]] |
| - | [[Category: Reddy | + | [[Category: Reddy VS]] |
| - | [[Category: Venkataraman | + | [[Category: Venkataraman S]] |
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Current revision
Crystal Structure of Ad16 fiber knob
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