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1dks

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(New page: 200px<br /> <applet load="1dks" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dks, resolution 3.2&Aring;" /> '''CKSHS1: HUMAN CYCLIN...)
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[[Image:1dks.gif|left|200px]]<br />
 
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<applet load="1dks" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dks, resolution 3.2&Aring;" />
 
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'''CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 IN COMPLEX WITH PHOSPHATE'''<br />
 
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==Overview==
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==CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 IN COMPLEX WITH PHOSPHATE==
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The structure of the human CksHs1 homolog of the yeast cell-cycle, regulatory proteins suc1 and CKS1, which bind to the catalytic subunit of, the cyclin-dependent kinases (Cdks) and are essential for yeast cell-cycle, progression in vivo, has been determined at 2.9 A resolution. The CksHs1, single polypeptide domain fold, which consists of a four-stranded, beta-sheet flanked by two alpha-helices, is dramatically different from, the subunit conformation and assembly of the homologous CksHs2, but, strikingly similar to the Cdk N-lobe domain fold. The CksHs1 structure, identifies sequence-conserved residues Glu61 to His65 as a novel, beta-hinge region that folds back to form a beta-hairpin with CksHs1, subunit, whereas this hinge is unfolded to form an extended beta-strand, exchange between two CksHs2 subunits. Phosphate and the phosphate analog, metavanadate bind CksHs1 in a shallow pocket and interact with five, conserved residues (Lys11, Arg20, Ser51, Trp54 and Arg71) suggesting a, specific Cks recognition site for a phosphorylated Cdk residue. The, dramatic changes to the Cks fold, assembly and exposed conserved surface, brought about by switching between the bent and extended hinge, conformations are potentially important for the functions of this Cks, homolog and could explain conflicting activities inferred from different, types of genetic experiments.
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<StructureSection load='1dks' size='340' side='right'caption='[[1dks]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DKS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dks OCA], [https://pdbe.org/1dks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dks RCSB], [https://www.ebi.ac.uk/pdbsum/1dks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dks ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CKS1_HUMAN CKS1_HUMAN] Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dk/1dks_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dks ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DKS OCA].
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*[[Cyclin-dependent kinase 3D structures|Cyclin-dependent kinase 3D structures]]
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*[[Cyclin-dependent kinase regulatory subunit|Cyclin-dependent kinase regulatory subunit]]
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==Reference==
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*[[Cyclin-dependent kinase regulatory subunit 3D structures|Cyclin-dependent kinase regulatory subunit 3D structures]]
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Crystal structure of the human cell cycle protein CksHs1: single domain fold with similarity to kinase N-lobe domain., Arvai AS, Bourne Y, Hickey MJ, Tainer JA, J Mol Biol. 1995 Jun 23;249(5):835-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7791211 7791211]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arvai, A.S.]]
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[[Category: Arvai AS]]
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[[Category: Bourne, Y.]]
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[[Category: Bourne Y]]
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[[Category: Tainer, J.A.]]
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[[Category: Tainer JA]]
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[[Category: PO4]]
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[[Category: cell division]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:33:26 2007''
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Current revision

CKSHS1: HUMAN CYCLIN DEPENDENT KINASE SUBUNIT, TYPE 1 IN COMPLEX WITH PHOSPHATE

PDB ID 1dks

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