7opy
From Proteopedia
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==Camel GSTM1-1 in complex with S-(p-nitrobenzyl)glutathione== | ==Camel GSTM1-1 in complex with S-(p-nitrobenzyl)glutathione== | ||
| - | <StructureSection load='7opy' size='340' side='right'caption='[[7opy]]' scene=''> | + | <StructureSection load='7opy' size='340' side='right'caption='[[7opy]], [[Resolution|resolution]] 2.05Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OPY FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OPY FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7opy OCA], [https://pdbe.org/7opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7opy RCSB], [https://www.ebi.ac.uk/pdbsum/7opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7opy ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GTB:S-(P-NITROBENZYL)GLUTATHIONE'>GTB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7opy OCA], [https://pdbe.org/7opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7opy RCSB], [https://www.ebi.ac.uk/pdbsum/7opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7opy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glutathione transferases (GSTs; EC. 2.5.1.18) are a large family of multifunctional enzymes that play crucial roles in the metabolism and inactivation of a broad range of xenobiotic compounds. In the present work, we report the kinetic and structural characterization of the isoenzyme GSTM1-1 from Camelus dromedarius (CdGSTM1-1). The CdGSTauM1-1 was expressed in E. coli BL21 (DE3) and was purified by affinity chromatography. Kinetics analysis showed that the enzyme displays a relative narrow substrate specificity and restricted ability to bind xenobiotic compounds. The crystal structures of CdGSTauM1-1 were determined by X-ray crystallography in complex with the substrate (GSH) or the reaction product (S-p-nitrobenzyl-GSH), providing snapshots of the induced-fit catalytic mechanism. The thermodynamic stability of CdGSTM1-1 was investigated using differential scanning fluorimetry (DSF) in the absence and in presence of GSH and S-p-nitrobenzyl-GSH and revealed that the enzyme's structure is significantly stabilized by its ligands. The results of the present study advance the understanding of camelid GST detoxification mechanisms and their contribution to abiotic stress adaptation in harsh desert conditions. | ||
| + | |||
| + | Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1.,Perperopoulou F, Poudel N, Papageorgiou AC, Ataya FS, Labrou NE Life (Basel). 2022 Jan 12;12(1). pii: life12010106. doi: 10.3390/life12010106. PMID:35054499<ref>PMID:35054499</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7opy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Camel GSTM1-1 in complex with S-(p-nitrobenzyl)glutathione
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