2lus

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==NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity==
==NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity==
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<StructureSection load='2lus' size='340' side='right'caption='[[2lus]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2lus' size='340' side='right'caption='[[2lus]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2lus]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carro Carro]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2lus]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Carcinoscorpius_rotundicauda Carcinoscorpius rotundicauda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUS FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lus OCA], [https://pdbe.org/2lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lus RCSB], [https://www.ebi.ac.uk/pdbsum/2lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lus ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lus OCA], [https://pdbe.org/2lus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lus RCSB], [https://www.ebi.ac.uk/pdbsum/2lus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lus ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A3E0V9_CARRO A3E0V9_CARRO]
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Thioredoxins (Trxs), which play a key role in maintaining a redox environment in the cell, are found in almost all organisms. Trxs act as potential reducing agents of disulfide bonds and contain two vicinal cysteines in a CXXC motif at the active site. Trx is also known to activate the DNA binding activity of NF-kappaB, an important transcription factor. Previously, Trx-related protein 16 from Carcinoscorpius rotundicauda (Cr-TRP16), a 16-kDa Trx-like protein that contains a WCPPC motif, was reported. Here we present the NMR structure of the reduced form of Cr-TRP16, along with its regulation of NF-kappaB activity. Unlike other 16-kDa Trx-like proteins, Cr-TRP16 contains an additional Cys residue (Cys-15, at the N terminus), through which it forms a homodimer. Moreover, we have explored the molecular basis of Cr-TRP16-mediated activation of NF-kappaB and showed that Cr-TRP16 exists as a dimer under physiological conditions, and only the dimeric form binds to NF-kappaB and enhances its DNA binding activity by directly reducing the cysteines in the DNA-binding motif of NF-kappaB. The C15S mutant of Cr-TRP16 was unable to dimerize and hence does not bind to NF-kappaB. Based on our finding and combined with the literature, we propose a model of how Cr-TRP16 is likely to bind to NF-kappaB. These findings elucidate the molecular mechanism by which NF-kappaB activation is regulated through Cr-TRP16.
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NMR structure of Carcinoscorpius rotundicauda thioredoxin-related protein 16 and its role in regulating transcription factor NF-kappaB activity.,Giri PK, Jing-Song F, Shanmugam MK, Ding JL, Sethi G, Swaminathan K, Sivaraman J J Biol Chem. 2012 Aug 24;287(35):29417-28. doi: 10.1074/jbc.M112.379859. Epub, 2012 Jul 3. PMID:22763700<ref>PMID:22763700</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2lus" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Carro]]
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[[Category: Carcinoscorpius rotundicauda]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Fan, J]]
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[[Category: Fan J]]
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[[Category: Giri, P K]]
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[[Category: Giri PK]]
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[[Category: Kunchithapadam, S]]
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[[Category: Kunchithapadam S]]
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[[Category: Sivaraman, J]]
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[[Category: Sivaraman J]]
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[[Category: Cr-trp16]]
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[[Category: Oxidoreductase]]
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Current revision

NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity

PDB ID 2lus

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