2lwm

<StructureSection load='2lwm' size='340' side='right'caption='[[2lwm]], [[NMR_Ensembles_of_Models | 26 NMR models]]' scene=''>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LWM:[(1R,2S,4R)-4-{[2-AMINO-5-(FORMYLAMINO)-6-OXO-1,6-DIHYDROPYRIMIDIN-4-YL]AMINO}-2-HYDROXYCYCLOPENTYL]METHYL+DIHYDROGEN+PHOSPHATE'>LWM</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lwn|2lwn]], [[2lwo|2lwo]]</div></td></tr>

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== Publication Abstract from PubMed ==

The addition of hydroxyl radicals to the C8 position of guanine can lead to the formation of a 2,6-diamino-4-hydroxy-5-formamido-2'-deoxypyrimidine (Fapy-dG) lesion, whose endogenous levels in cellular DNA rival those of 8-oxo-7,8-dihydroxy-2'-deoxyguanosine. Despite its prevalence, the structure of duplex DNA containing Fapy-dG is unknown. We have prepared an undecameric duplex containing a centrally located beta-cFapy-dG residue paired to dC and determined its solution structure by high-resolution NMR spectroscopy and restrained molecular dynamic simulations. The damaged duplex adopts a right-handed helical structure with all residues in an anti conformation, forming Watson-Crick base pair alignments, and 2-deoxyribose conformations in the C2'-endo/C1'-exo range. The formamido group of Fapy rotates out of the pyrimidine plane and is present in the Z and E configurations that equilibrate with an approximate 2:1 population ratio. The two isomeric duplexes show similar lesion-induced deviations from a canonical B-from DNA conformation that are minor and limited to the central three-base-pair segment of the duplex, affecting the stacking interactions with the 5-lesion-neighboring residue. We discuss the implications of our observations for translesion synthesis during DNA replication and the recognition of Fapy-dG by DNA glycosylases.

Solution structure of duplex DNA containing a beta-Carba-Fapy-dG lesion.,Lukin M, Zaliznyak T, Attaluri S, Johnson F, de Los Santos C Chem Res Toxicol. 2012 Nov 19;25(11):2423-31. doi: 10.1021/tx300290b. Epub 2012, Aug 29. PMID:22897814<ref>PMID:22897814</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2lwm" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Attaluri, S]]

[[Category: Johnson, F]]

[[Category: Lukin, M]]

[[Category: Santos, C de los]]

[[Category: Zalianyak, T]]

[[Category: Dna]]

[[Category: Oxidative damage]]