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2mj0

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Current revision (11:56, 22 November 2023) (edit) (undo)
 
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==Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia==
==Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia==
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<StructureSection load='2mj0' size='340' side='right'caption='[[2mj0]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2mj0' size='340' side='right'caption='[[2mj0]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Monocled_cobra Monocled cobra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJ0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_kaouthia Naja kaouthia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJ0 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mj0 OCA], [https://pdbe.org/2mj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mj0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mj0 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mj0 OCA], [https://pdbe.org/2mj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mj0 RCSB], [https://www.ebi.ac.uk/pdbsum/2mj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mj0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/TXWW_NAJKA TXWW_NAJKA]] Modulates both nicotinic and muscarinic acetylcholine receptors (nAChR and mAChR/CHRM). On nAChR, acts as an orthosteric (competitive) antagonist. On mAChR, in presence of ACh, it partially inhibits the effect of ACh (allosteric antagonist), whereas in the absence of ACh, it activates the receptor (allosteric agonist). Exerts a myorelaxant effect, induces a dose-dependent decrease in blood pressure and an increase in heart rate in mice and rats.<ref>PMID:11279130</ref> <ref>PMID:15581687</ref> <ref>PMID:17371532</ref> <ref>PMID:19682302</ref>
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[https://www.uniprot.org/uniprot/3NO2_NAJKA 3NO2_NAJKA] Neurotoxin that irreversibly inhibits nicotinic acetylcholine receptors (nAChR) and allosterically interacts with muscarinic acetylcholine receptors (mAChR) (PubMed:11279130, PubMed:19682302). The loop II is involved in the interaction of this toxin with nAChR and mAChR (PubMed:27343701, PubMed:26242733). On nAChR, it acts as a competitive antagonist (muscle-type and alpha-7/CHRNA7) with IC(50) values in the micromolar range (PubMed:11279130, PubMed:27343701). On mAChR, in presence of ACh, it partially inhibits the effect of acetylcholine (ACh) (allosteric antagonist), whereas in the absence of ACh, it activates the receptor (allosteric agonist) (PubMed:19682302). It also shows a very weak inhibition of GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1 and GABRB3 and GABRG2) subunits (10 uM inhibit 31% current) (PubMed:26221036). In vivo, is nonlethal to mice at concentrations up to 20 mg/kg, but exerts a myorelaxant effect, induces a dose-dependent decrease in blood pressure and an increase in heart rate in mice and rats (PubMed:15581687, PubMed:17371532).<ref>PMID:11279130</ref> <ref>PMID:15581687</ref> <ref>PMID:17371532</ref> <ref>PMID:19682302</ref> <ref>PMID:26221036</ref> <ref>PMID:27343701</ref>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Monocled cobra]]
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[[Category: Naja kaouthia]]
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[[Category: Lyukmanova, E N]]
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[[Category: Lyukmanova EN]]
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[[Category: Paramonov, A S]]
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[[Category: Paramonov AS]]
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[[Category: Shenkarev, Z O]]
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[[Category: Shenkarev ZO]]
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[[Category: Muscarinic acetylcholine receptor]]
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[[Category: Nicotonic acetylcholine receptor]]
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[[Category: Non-conventional toxin]]
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[[Category: Toxin]]
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[[Category: Weak toxin]]
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[[Category: Wtx]]
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Current revision

Spatial structure of P33A mutant of non-conventional toxin WTX from Naja kaouthia

PDB ID 2mj0

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