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1gfl

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[[Image:1gfl.gif|left|200px]]
 
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==STRUCTURE OF GREEN FLUORESCENT PROTEIN==
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The line below this paragraph, containing "STRUCTURE_1gfl", creates the "Structure Box" on the page.
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<StructureSection load='1gfl' size='340' side='right'caption='[[1gfl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gfl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The June 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Green Fluorescent Protein (GFP)'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_6 10.2210/rcsb_pdb/mom_2003_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GFL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gfl OCA], [https://pdbe.org/1gfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gfl RCSB], [https://www.ebi.ac.uk/pdbsum/1gfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gfl ProSAT]</span></td></tr>
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{{STRUCTURE_1gfl| PDB=1gfl | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/1gfl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gfl ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF GREEN FLUORESCENT PROTEIN'''
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==See Also==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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==Overview==
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__TOC__
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The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been solved to a resolution of 1.9 A by multiwavelength anomalous dispersion phasing methods. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. This motif, with beta-structure on the outside and alpha-helix on the inside, represents a new protein fold, which we have named the beta-can. Two protomers pack closely together to form a dimer in the crystal. The fluorophores are protected inside the cylinders, and their structures are consistent with the formation of aromatic systems made up of Tyr66 with reduction of its C alpha-C beta bond coupled with cyclization of the neighboring glycine and serine residues. The environment inside the cylinder explains the effects of many existing mutants of GFP and suggests specific side chains that could be modified to change the spectral properties of GFP. Furthermore, the identification of the dimer contacts may allow mutagenic control of the state of assembly of the protein.
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</StructureSection>
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==About this Structure==
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1GFL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. The following page contains interesting information on the relation of 1GFL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb42_1.html Green Fluorescent Protein (GFP)]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GFL OCA].
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==Reference==
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The molecular structure of green fluorescent protein., Yang F, Moss LG, Phillips GN Jr, Nat Biotechnol. 1996 Oct;14(10):1246-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9631087 9631087]
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jr., G N.Phillips.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Moss, L G.]]
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[[Category: Moss LG]]
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[[Category: Yang, F.]]
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[[Category: Phillips Jr GN]]
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[[Category: Fluorophore green fluorescent protein]]
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[[Category: Yang F]]
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[[Category: Luminescence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:30:14 2008''
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Current revision

STRUCTURE OF GREEN FLUORESCENT PROTEIN

PDB ID 1gfl

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