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| | ==Solution Structure of the Anti-Sigma Factor AsiA Homodimer== | | ==Solution Structure of the Anti-Sigma Factor AsiA Homodimer== |
| - | <StructureSection load='1jr5' size='340' side='right'caption='[[1jr5]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='1jr5' size='340' side='right'caption='[[1jr5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jr5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JR5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jr5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JR5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asiA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr5 OCA], [https://pdbe.org/1jr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jr5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jr5 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr5 OCA], [https://pdbe.org/1jr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jr5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jr5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ASIA_BPT4 ASIA_BPT4]] Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.<ref>PMID:8021178</ref> <ref>PMID:15257291</ref>
| + | [https://www.uniprot.org/uniprot/ASIA_BPT4 ASIA_BPT4] Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.<ref>PMID:8021178</ref> <ref>PMID:15257291</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adelman, K]] | + | [[Category: Adelman K]] |
| - | [[Category: Brody, E N]] | + | [[Category: Bieber Urbauer RJ]] |
| - | [[Category: Gilmore, J M]] | + | [[Category: Brody EN]] |
| - | [[Category: Simeonov, M F]] | + | [[Category: Gilmore JM]] |
| - | [[Category: Urbauer, J L]] | + | [[Category: Simeonov MF]] |
| - | [[Category: Urbauer, R J.Bieber]] | + | [[Category: Urbauer JL]] |
| - | [[Category: All-alpha]]
| + | |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
ASIA_BPT4 Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Anti-sigma factors regulate prokaryotic gene expression through interactions with specific sigma factors. The bacteriophage T4 anti-sigma factor AsiA is a molecular switch that both inhibits transcription from bacterial promoters and phage early promoters and promotes transcription at phage middle promoters through its interaction with the primary sigma factor of Escherichia coli, sigma(70). AsiA is an all-helical, symmetric dimer in solution. The solution structure of the AsiA dimer reveals a novel helical fold for the protomer. Furthermore, the AsiA protomer, surprisingly, contains a helix-turn-helix DNA binding motif, predicting a potential new role for AsiA. The AsiA dimer interface includes a substantial hydrophobic component, and results of hydrogen/deuterium exchange studies suggest that the dimer interface is the most stable region of the AsiA dimer. In addition, the residues that form the dimer interface are those that are involved in binding to sigma(70). The results promote a model whereby the AsiA dimer maintains the active hydrophobic surfaces and delivers them to sigma(70), where an AsiA protomer is displaced from the dimer via the interaction of sigma(70) with the same residues in AsiA that constitute the dimer interface.
Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions.,Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1831-5. Epub 2002 Feb 5. PMID:11830637[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ouhammouch M, Orsini G, Brody EN. The asiA gene product of bacteriophage T4 is required for middle mode RNA synthesis. J Bacteriol. 1994 Jul;176(13):3956-65. PMID:8021178
- ↑ Lambert LJ, Wei Y, Schirf V, Demeler B, Werner MH. T4 AsiA blocks DNA recognition by remodeling sigma70 region 4. EMBO J. 2004 Aug 4;23(15):2952-62. Epub 2004 Jul 15. PMID:15257291 doi:10.1038/sj.emboj.7600312
- ↑ Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN. Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1831-5. Epub 2002 Feb 5. PMID:11830637 doi:10.1073/pnas.032464699
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