1lwr

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of the NCAM fibronectin type III module 2

Structural highlights

1lwr is a 1 chain structure with sequence from Rattus norvegicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAM1_RAT This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The neural cell adhesion molecule (NCAM) promotes axonal outgrowth, presumably through an interaction with the fibroblast growth factor receptor (FGFR). NCAM also has a little-understood ATPase activity. We here demonstrate for the first time a direct interaction between NCAM (fibronectin type III [F3] modules 1 and 2) and FGFR1 (Ig modules 2 and 3) by surface plasmon resonance (SPR) analysis. The structure of the NCAM F3 module 2 was determined by NMR and the module was shown by NMR to interact with the FGFR1 Ig module 3 and ATP. The NCAM sites binding to FGFR and ATP were found to overlap and ATP was shown by SPR to inhibit the NCAM-FGFR binding, indicating that ATP probably regulates the NCAM-FGFR interaction. Furthermore, we demonstrate that the NCAM module was able to induce activation (phosphorylation) of FGFR and to stimulate neurite outgrowth. In contrast, ATP inhibited neurite outgrowth induced by the module.

Structural basis for a direct interaction between FGFR1 and NCAM and evidence for a regulatory role of ATP.,Kiselyov VV, Skladchikova G, Hinsby AM, Jensen PH, Kulahin N, Soroka V, Pedersen N, Tsetlin V, Poulsen FM, Berezin V, Bock E Structure. 2003 Jun;11(6):691-701. PMID:12791257^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kiselyov VV, Skladchikova G, Hinsby AM, Jensen PH, Kulahin N, Soroka V, Pedersen N, Tsetlin V, Poulsen FM, Berezin V, Bock E. Structural basis for a direct interaction between FGFR1 and NCAM and evidence for a regulatory role of ATP. Structure. 2003 Jun;11(6):691-701. PMID:12791257

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lwr"

@@ Line 1: / Line 1: @@
 ==Solution structure of the NCAM fibronectin type III module 2==
-<StructureSection load='1lwr' size='340' side='right'caption='[[1lwr]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+<StructureSection load='1lwr' size='340' side='right'caption='[[1lwr]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1lwr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LWR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1lwr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LWR FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ncam1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lwr OCA], [https://pdbe.org/1lwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lwr RCSB], [https://www.ebi.ac.uk/pdbsum/1lwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lwr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/NCAM1_RAT NCAM1_RAT]] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
+[https://www.uniprot.org/uniprot/NCAM1_RAT NCAM1_RAT] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Buffalo rat]]
 [[Category: Large Structures]]
-[[Category: Berezin, V]]
+[[Category: Rattus norvegicus]]
-[[Category: Bock, E]]
+[[Category: Berezin V]]
-[[Category: Hinsby, A M]]
+[[Category: Bock E]]
-[[Category: Jensen, P H]]
+[[Category: Hinsby AM]]
-[[Category: Kiselyov, V V]]
+[[Category: Jensen PH]]
-[[Category: Kulahin, N]]
+[[Category: Kiselyov VV]]
-[[Category: Pedersen, N]]
+[[Category: Kulahin N]]
-[[Category: Poulsen, F M]]
+[[Category: Pedersen N]]
-[[Category: Skladchikova, G]]
+[[Category: Poulsen FM]]
-[[Category: Tsetlin, V]]
+[[Category: Skladchikova G]]
-[[Category: All beta]]
+[[Category: Tsetlin V]]
-[[Category: Cell adhesion]]
-[[Category: Fibronectin type iii module]]

1lwr

From Proteopedia

Current revision

Solution structure of the NCAM fibronectin type III module 2

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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