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| | ==Solution structure of thiopurine methyltransferase from Pseudomonas syringae== | | ==Solution structure of thiopurine methyltransferase from Pseudomonas syringae== |
| - | <StructureSection load='1pjz' size='340' side='right'caption='[[1pjz]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1pjz' size='340' side='right'caption='[[1pjz]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1pjz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psesj Psesj]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1pjz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._pisi Pseudomonas syringae pv. pisi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJZ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TPM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59510 PSESJ])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiopurine_S-methyltransferase Thiopurine S-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.67 2.1.1.67] </span></td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjz OCA], [https://pdbe.org/1pjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjz RCSB], [https://www.ebi.ac.uk/pdbsum/1pjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjz OCA], [https://pdbe.org/1pjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjz RCSB], [https://www.ebi.ac.uk/pdbsum/1pjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TPMT_PSESJ TPMT_PSESJ]] Involved in the biological cycling of tellurium and selenium. Tellurium resistance (Ter) mechanism.
| + | [https://www.uniprot.org/uniprot/TPMT_PSESJ TPMT_PSESJ] Involved in the biological cycling of tellurium and selenium. Tellurium resistance (Ter) mechanism. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Psesj]] | + | [[Category: Pseudomonas syringae pv. pisi]] |
| - | [[Category: Thiopurine S-methyltransferase]]
| + | [[Category: Hodsdon ME]] |
| - | [[Category: Hodsdon, M E]] | + | [[Category: Lolis E]] |
| - | [[Category: Lolis, E]] | + | [[Category: Scheuermann TH]] |
| - | [[Category: Scheuermann, T H]] | + | |
| - | [[Category: Drug metabolism]]
| + | |
| - | [[Category: Methyltransferase]]
| + | |
| - | [[Category: Polymorphism]]
| + | |
| - | [[Category: S-adenosylmethionine]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
TPMT_PSESJ Involved in the biological cycling of tellurium and selenium. Tellurium resistance (Ter) mechanism.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In humans, the enzyme thiopurine methyltransferase (TPMT) metabolizes 6-thiopurine (6-TP) medications, including 6-thioguanine, 6-mercaptopurine and azathioprine, commonly used for immune suppression and for the treatment of hematopoietic malignancies. S-Methylation by TPMT prevents the intracellular conversion of these drugs into active 6-thioguanine nucleotides (6-TGNs). Genetic polymorphisms in the TPMT protein sequence have been associated with decreased tissue enzymatic activities and an increased risk of life-threatening myelo-suppression from standard doses of 6-TP medications. Biochemical studies have demonstrated that TPMT deficiency is primarily associated with increased degradation of the polymorphic proteins through an ubiquitylation and proteasomal-dependent pathway. We have now determined the tertiary structure of the bacterial orthologue of TPMT from Pseudomonas syringae using NMR spectroscopy. Bacterial TPMT similarly catalyzes the S-adenosylmethionine (SAM)-dependent transmethylation of 6-TPs and shares 45% similarity (33% identity) with the human enzyme. Initial studies revealed an unstructured N terminus, which was removed for structural studies and subsequently determined to be required for enzymatic activity. Despite lacking sequence similarity to any protein of known three-dimensional structure, the tertiary structure of bacterial TPMT reveals a classical SAM-dependent methyltransferase topology, consisting of a seven-stranded beta-sheet flanked by alpha-helices on both sides. However, some deviations from the consensus topology, along with multiple insertions of structural elements, are evident. A review of the many experimentally determined tertiary structures of SAM-dependent methyltransferases demonstrates that such structural deviations from the consensus topology are common and often functionally important.
Tertiary structure of thiopurine methyltransferase from Pseudomonas syringae, a bacterial orthologue of a polymorphic, drug-metabolizing enzyme.,Scheuermann TH, Lolis E, Hodsdon ME J Mol Biol. 2003 Oct 24;333(3):573-85. PMID:14556746[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Scheuermann TH, Lolis E, Hodsdon ME. Tertiary structure of thiopurine methyltransferase from Pseudomonas syringae, a bacterial orthologue of a polymorphic, drug-metabolizing enzyme. J Mol Biol. 2003 Oct 24;333(3):573-85. PMID:14556746
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