3eu1

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution

Structural highlights

3eu1 is a 4 chain structure with sequence from Capra hircus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HBA1_CAPHI Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.[UniProtKB:P01946]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Hemoglobin is a vital protein present in almost all higher species. It is a transport protein involved in carrying oxygen from lungs to tissues and carbon dioxide back to lungs by an intrinsically coordinated manner. Even though a good amount of work has been carried out in this direction there exists scarcity of structural insight on low oxygen affinity species. Attempts are being made to unravel the structural insight of this low oxygen affinity species. Goat blood plasma was collected, treated with EDTA to avoid blood clotting and purification was accomplished using DEAE-anion chromatographic column. The goat hemoglobin was crystallized using 50mM of phosphate buffer at pH 6.7 with 1M NaCl and PEG 3350 as precipitant by hanging drop vapor diffusion method. Crystals obtained are screened and suitable crystals are taken for data collection using mar345dtb as image plate detector system. Goat hemoglobin crystal diffracted up to 2.61 A resolution. Goat hemoglobin crystallizes in orthorhombic space group P212(1)2(1) as a whole biological molecule in the asymmetric unit with cell dimensions a=53.568A, b=67.365A, c=154.183A.

Purification, Crystallization and Preliminary X-Ray Diffraction Studies on Goat (Capra hircus) Hemoglobin - A Low Oxygen Affinity Species.,Moorthy PS, Neelagandan K, Balasubramanian M, Ponnuswamy MN Protein Pept Lett. 2009;16(4):454-6. PMID:19356147^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moorthy PS, Neelagandan K, Balasubramanian M, Ponnuswamy MN. Purification, Crystallization and Preliminary X-Ray Diffraction Studies on Goat (Capra hircus) Hemoglobin - A Low Oxygen Affinity Species. Protein Pept Lett. 2009;16(4):454-6. PMID:19356147

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3eu1"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[3eu1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EU1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qu0|2qu0]], [[2ri4|2ri4]], [[3d1a|3d1a]], [[3cy5|3cy5]], [[3d4x|3d4x]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eu1 OCA], [https://pdbe.org/3eu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eu1 RCSB], [https://www.ebi.ac.uk/pdbsum/3eu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eu1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/HBBA_CAPHI HBBA_CAPHI]] Involved in oxygen transport from the lung to the various peripheral tissues.
+[https://www.uniprot.org/uniprot/HBA1_CAPHI HBA1_CAPHI] Involved in oxygen transport from the lung to the various peripheral tissues.  Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.[UniProtKB:P01946]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 38: / Line 38: @@
 [[Category: Capra hircus]]
 [[Category: Large Structures]]
-[[Category: Balasubramanian, M]]
+[[Category: Balasubramanian M]]
-[[Category: Moorthy, P Sathya]]
+[[Category: Neelagandan K]]
-[[Category: Neelagandan, K]]
+[[Category: Ponnuswamy MN]]
-[[Category: Ponnuswamy, M N]]
+[[Category: Sathya Moorthy P]]
-[[Category: 3-diphospho glycerate]]
-[[Category: Allosteric mechanism]]
-[[Category: Hemoglobin]]
-[[Category: Low oxygen affinity]]
-[[Category: Oxygen storage]]
-[[Category: Oxygen transport]]

3eu1

From Proteopedia

Current revision

Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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