7q84
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==Crystal structure of human peroxisomal acyl-Co-A oxidase 1a, apo-form== | ==Crystal structure of human peroxisomal acyl-Co-A oxidase 1a, apo-form== | ||
| - | <StructureSection load='7q84' size='340' side='right'caption='[[7q84]]' scene=''> | + | <StructureSection load='7q84' size='340' side='right'caption='[[7q84]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q84 FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q84 FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q84 OCA], [https://pdbe.org/7q84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q84 RCSB], [https://www.ebi.ac.uk/pdbsum/7q84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q84 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q84 OCA], [https://pdbe.org/7q84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q84 RCSB], [https://www.ebi.ac.uk/pdbsum/7q84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q84 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Peroxisomal acyl-CoA oxidase 1a (ACOX1a) catalyzes the first and rate-limiting step of fatty acid oxidation, the conversion of acyl-CoAs to 2-trans-enoyl-CoAs. The dysfunction of human ACOX1a (hACOX1a) leads to deterioration of the nervous system manifesting in myeloneuropathy, hypotonia and convulsions. Crystal structures of hACOX1a in apo- and cofactor (FAD)-bound forms were solved at 2.00 and 2.09 A resolution, respectively. hACOX1a exists as a homo-dimer with solvation free energy gain (DeltaG(o)) of -44.7 kcal mol(-1). Two FAD molecules bind at the interface of protein monomers completing the active sites. The substrate binding cleft of hACOX1a is wider compared to human mitochondrial very-long chain specific acyl-CoA dehydrogenase. Mutations (p.G178C, p.M278V and p.N237S) reported to cause dysfunctionality of hACOX1a are analyzed on its 3D-structure to understand structure-function related perturbations and explain the associated phenotypes. | ||
| + | |||
| + | Crystal structures of apo- and FAD-bound human peroxisomal acyl-CoA oxidase provide mechanistic basis explaining clinical observations.,Sonani RR, Blat A, Dubin G Int J Biol Macromol. 2022 Feb 8;205:203-210. doi: 10.1016/j.ijbiomac.2022.02.008. PMID:35149097<ref>PMID:35149097</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7q84" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of human peroxisomal acyl-Co-A oxidase 1a, apo-form
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