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| | ==Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1== | | ==Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1== |
| - | <StructureSection load='1pxe' size='340' side='right'caption='[[1pxe]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | + | <StructureSection load='1pxe' size='340' side='right'caption='[[1pxe]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1pxe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PXE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1pxe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PXE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NZF-1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pxe OCA], [https://pdbe.org/1pxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pxe RCSB], [https://www.ebi.ac.uk/pdbsum/1pxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pxe ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pxe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pxe OCA], [https://pdbe.org/1pxe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pxe RCSB], [https://www.ebi.ac.uk/pdbsum/1pxe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pxe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/MYT1L_RAT MYT1L_RAT]] May function as a panneural transcription factor associated with neuronal differentiation. May play a role in the development of neurons and oligodendrogalia in the CNS.<ref>PMID:9373037</ref>
| + | [https://www.uniprot.org/uniprot/MYT1L_RAT MYT1L_RAT] May function as a panneural transcription factor associated with neuronal differentiation. May play a role in the development of neurons and oligodendrogalia in the CNS.<ref>PMID:9373037</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Amann, B T]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Berg, J M]] | + | [[Category: Amann BT]] |
| - | [[Category: Berkovits-Cymet, H J]] | + | [[Category: Berg JM]] |
| - | [[Category: Cchhc zinc binding domain]] | + | [[Category: Berkovits-Cymet HJ]] |
| - | [[Category: Dna binding domain]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Neural zinc finger factor-1]]
| + | |
| Structural highlights
Function
MYT1L_RAT May function as a panneural transcription factor associated with neuronal differentiation. May play a role in the development of neurons and oligodendrogalia in the CNS.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 (NZF-1) has been determined in solution though the use of NMR methods. This domain is a member of a family of domains that have the Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure determination reveals a novel fold based around a zinc(II) ion coordinated to three Cys residues and the second of the two conserved His residues. The other His residue is stacked between the metal-coordinated His residue and a relatively conserved aromatic residue. Analysis of His to Gln sequence variants reveals that both His residues are required for the formation of a well-defined structure, but neither is required for high-affinity metal binding at a tetrahedral site. The structure suggests that a two-domain protein fragment and a double-stranded DNA binding site may interact with a common two-fold axis relating the two domains and the two half-sites of the DNA-inverted repeat.
Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding.,Berkovits-Cymet HJ, Amann BT, Berg JM Biochemistry. 2004 Feb 3;43(4):898-903. PMID:14744132[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. J Neurosci Res. 1997 Oct 15;50(2):272-90. PMID:9373037
- ↑ Berkovits-Cymet HJ, Amann BT, Berg JM. Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding. Biochemistry. 2004 Feb 3;43(4):898-903. PMID:14744132 doi:10.1021/bi035159d
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