1rzs

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of P22 Cro

Structural highlights

1rzs is a 1 chain structure with sequence from Salmonella virus P22. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RCRO_BPP22 Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the solution structure of the Cro protein from bacteriophage P22. Comparisons of its sequence and structure to those of lambda Cro strongly suggest an alpha-to-beta secondary structure switching event during Cro evolution. The folds of P22 Cro and lambda Cro share a three alpha helix fragment comprising the N-terminal half of the domain. However, P22 Cro's C terminus folds as two helices, while lambda Cro's folds as a beta hairpin. The all-alpha fold found for P22 Cro appears to be ancestral, since it also occurs in cI proteins, which are anciently duplicated paralogues of Cro. PSI-BLAST and transitive homology analyses strongly suggest that the sequences of P22 Cro and lambda Cro are globally homologous despite encoding different folds. The alpha+beta fold of lambda Cro therefore likely evolved from its all-alpha ancestor by homologous secondary structure switching, rather than by nonhomologous replacement of both sequence and structure.

Secondary structure switching in Cro protein evolution.,Newlove T, Konieczka JH, Cordes MH Structure. 2004 Apr;12(4):569-81. PMID:15062080^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Newlove T, Konieczka JH, Cordes MH. Secondary structure switching in Cro protein evolution. Structure. 2004 Apr;12(4):569-81. PMID:15062080 doi:10.1016/j.str.2004.02.024

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rzs"

Categories: Large Structures | Salmonella virus P22 | Cordes MH | Konieczka JH | Newlove T

@@ Line 1: / Line 1: @@
 ==Solution structure of P22 Cro==
-<StructureSection load='1rzs' size='340' side='right'caption='[[1rzs]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''>
+<StructureSection load='1rzs' size='340' side='right'caption='[[1rzs]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1rzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1rzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZS FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CRO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzs OCA], [https://pdbe.org/1rzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rzs RCSB], [https://www.ebi.ac.uk/pdbsum/1rzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rzs ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RCRO_BPP22 RCRO_BPP22]] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
+[https://www.uniprot.org/uniprot/RCRO_BPP22 RCRO_BPP22] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bpp22]]
 [[Category: Large Structures]]
-[[Category: Cordes, M H]]
+[[Category: Salmonella virus P22]]
-[[Category: Konieczka, J H]]
+[[Category: Cordes MH]]
-[[Category: Newlove, T]]
+[[Category: Konieczka JH]]
-[[Category: Dna-binding protein]]
+[[Category: Newlove T]]
-[[Category: Helix-turn-helix]]
-[[Category: Structural evolution]]
-[[Category: Transcription]]

1rzs

From Proteopedia

Current revision

Solution structure of P22 Cro

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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