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| | <StructureSection load='6lfe' size='340' side='right'caption='[[6lfe]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='6lfe' size='340' side='right'caption='[[6lfe]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6lfe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LFE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lfe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LFE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EAO:[5-(2-ethanoyl-3-oxidanylidene-but-1-enyl)-2,3-bis(oxidanyl)phenyl]-oxidanyl-oxidanylidene-azanium'>EAO</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Comt ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EAO:3-(3,4-dihydroxy-5-nitrobenzylidene)pentane-2,4-dione'>EAO</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Catechol_O-methyltransferase Catechol O-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.6 2.1.1.6] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lfe OCA], [https://pdbe.org/6lfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lfe RCSB], [https://www.ebi.ac.uk/pdbsum/6lfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lfe ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lfe OCA], [https://pdbe.org/6lfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lfe RCSB], [https://www.ebi.ac.uk/pdbsum/6lfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lfe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/COMT_RAT COMT_RAT]] Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
| + | [https://www.uniprot.org/uniprot/COMT_RAT COMT_RAT] Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| - | [[Category: Catechol O-methyltransferase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Iijima, H]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Suzuki, M]] | + | [[Category: Iijima H]] |
| - | [[Category: Takebe, K]] | + | [[Category: Suzuki M]] |
| - | [[Category: Catecholamine]] | + | [[Category: Takebe K]] |
| - | [[Category: Enzyme s-adenosylmethionone catechol]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
6lfe is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.6Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
COMT_RAT Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Publication Abstract from PubMed
Catechol O-methyltransferase (COMT) is known as an important drug-target protein in the field of Parkinson's disease. All clinically approved COMT inhibitors bring a 5-substituted-3-nitrocatechol ring as a pharmacophore, and they bind to COMT with S-adenosylmethionine (SAM) and an Mg(2+) ion to form a quaternary complex (COMT/SAM/Mg(2+)/inhibitor). However, structural information about such quaternary complexes is only available for a few inhibitors. Here, a new crystal structure of COMT complexed with nitecapone (5), SAM and Mg(2+) is revealed. Comparison of the structures of these complexes indicates that conformation of the catechol binding pocket is almost constant regardless of structure of the inhibitors. The only restriction of the side chain of inhibitors (i.e., the substituent at the 5-position of 3-nitrocatechol) seems to be that it does not make steric repulsion with COMT. However, recent crystallographic and biochemical studies suggest that COMT is a flexible protein, and its conformational flexibility seems crucial for its catalytic process. Based on this information, implications of these quaternary inhibitor complexes were investigated. Met 40 in the alpha2alpha3-loop makes atomic contacts with SAM or S-adenosylhomocysteine and the 3-position of the catechol inhibitor. This interaction seems to play a critical role in the affinity of the inhibitor and to stabilize the COMT/SAM/Mg(2+)/nitrocatechol inhibitor complex by fixing the flexible alpha2alpha3-loop.
Crystal Structure of Catechol O-Methyltransferase Complexed with Nitecapone.,Iijima H, Takebe K, Suzuki M, Kobayashi H, Takamiya T, Saito H, Niwa N, Kuwada-Kusunose T Chem Pharm Bull (Tokyo). 2020;68(5):447-451. doi: 10.1248/cpb.c20-00011. PMID:32378542[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Iijima H, Takebe K, Suzuki M, Kobayashi H, Takamiya T, Saito H, Niwa N, Kuwada-Kusunose T. Crystal Structure of Catechol O-Methyltransferase Complexed with Nitecapone. Chem Pharm Bull (Tokyo). 2020;68(5):447-451. doi: 10.1248/cpb.c20-00011. PMID:32378542 doi:http://dx.doi.org/10.1248/cpb.c20-00011
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