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| | <StructureSection load='3foc' size='340' side='right'caption='[[3foc]], [[Resolution|resolution]] 2.09Å' scene=''> | | <StructureSection load='3foc' size='340' side='right'caption='[[3foc]], [[Resolution|resolution]] 2.09Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3foc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giaic Giaic]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3foc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FOC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GL50803_3032 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=184922 GIAIC])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3foc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3foc OCA], [https://pdbe.org/3foc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3foc RCSB], [https://www.ebi.ac.uk/pdbsum/3foc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3foc ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3foc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3foc OCA], [https://pdbe.org/3foc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3foc RCSB], [https://www.ebi.ac.uk/pdbsum/3foc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3foc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A8B8N3_GIAIC A8B8N3_GIAIC] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Giaic]] | + | [[Category: Giardia lamblia ATCC 50803]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Tryptophan--tRNA ligase]]
| + | [[Category: Arakaki TL]] |
| - | [[Category: Arakaki, T L]] | + | [[Category: Merritt EA]] |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Merritt, E A]] | + | |
| - | [[Category: Aminoacyl-trna synthetase]]
| + | |
| - | [[Category: Giardiasis]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Msgpp]]
| + | |
| - | [[Category: Tryptophanyl-trna synthetase]]
| + | |
| Structural highlights
Function
A8B8N3_GIAIC
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The 2.1A crystal structure of tryptophanyl-tRNA synthetase (TrpRS) from the diplomonad Giardia lamblia reveals that the N-terminus of this class I aminoacyl-tRNA synthetase forms a 16-residue alpha-helix. This helix replaces a beta-hairpin that is required by human TrpRS for normal activity and has been inferred to play a similar role in all eukaryotic TrpRS. The primary sequences of TrpRS homologs from several basal eukaryotes including Giardia lack a set of three residues observed to stabilize interactions with this beta-hairpin in the human TrpRS. Thus the present structure suggests that the activation reaction mechanism of TrpRS from the basal eukaryote G. lamblia differs from that of higher eukaryotes. Furthermore, the protein as observed in the crystal forms an (alpha(2))(2) homotetramer. The canonical dimer interface observed in all previous structures of tryptophanyl-tRNA synthetases is maintained, but in addition each N-terminal alpha-helix reciprocally interlocks with the equivalent helix from a second dimer to form a dimer of dimers. Although we have no evidence for tetramer formation in vivo, modeling indicates that the crystallographically observed tetrameric structure would be compatible with the tRNA binding mode used by dimeric TrpRS and TyrRS.
The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs.,Arakaki TL, Carter M, Napuli AJ, Verlinde CL, Fan E, Zucker F, Buckner FS, Van Voorhis WC, Hol WG, Merritt EA J Struct Biol. 2010 Aug;171(2):238-43. Epub 2010 May 8. PMID:20438846[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Arakaki TL, Carter M, Napuli AJ, Verlinde CL, Fan E, Zucker F, Buckner FS, Van Voorhis WC, Hol WG, Merritt EA. The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs. J Struct Biol. 2010 Aug;171(2):238-43. Epub 2010 May 8. PMID:20438846 doi:10.1016/j.jsb.2010.04.010
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