2pwt

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene></td></tr>

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== Publication Abstract from PubMed ==

The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.

Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity.,Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:17722211<ref>PMID:17722211</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2pwt" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Francois, B]]

[[Category: Hanessian, S]]

[[Category: Kondo, J]]

[[Category: Pachamuthu, K]]

[[Category: Szychowski, J]]

[[Category: Westhof, E]]

[[Category: X-ray analysis]]