1gku

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[[Image:1gku.gif|left|200px]]
 
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==Reverse gyrase from Archaeoglobus fulgidus==
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The line below this paragraph, containing "STRUCTURE_1gku", creates the "Structure Box" on the page.
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<StructureSection load='1gku' size='340' side='right'caption='[[1gku]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gku]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GKU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gku OCA], [https://pdbe.org/1gku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gku RCSB], [https://www.ebi.ac.uk/pdbsum/1gku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gku ProSAT]</span></td></tr>
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{{STRUCTURE_1gku| PDB=1gku | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RGYR_ARCFU RGYR_ARCFU] Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gku_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gku ConSurf].
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<div style="clear:both"></div>
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'''REVERSE GYRASE FROM ARCHAEOGLOBUS FULGIDUS'''
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==See Also==
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*[[Gyrase 3D Structures|Gyrase 3D Structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Reverse gyrase is the only topoisomerase known to positively supercoil DNA. The protein appears to be unique to hyperthermophiles, where its activity is believed to protect the genome from denaturation. The 120 kDa enzyme is the only member of the type I topoisomerase family that requires ATP, which is bound and hydrolysed by a helicase-like domain. We have determined the crystal structure of reverse gyrase from Archaeoglobus fulgidus in the presence and absence of nucleotide cofactor. The structure provides the first view of an intact supercoiling enzyme, explains mechanistic differences from other type I topoisomerases and suggests a model for how the two domains of the protein cooperate to positively supercoil DNA. Coordinates have been deposited in the Protein Data Bank under accession codes 1GKU and 1GL9.
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Rodriguez AC]]
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1GKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKU OCA].
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[[Category: Stock D]]
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==Reference==
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Crystal structure of reverse gyrase: insights into the positive supercoiling of DNA., Rodriguez AC, Stock D, EMBO J. 2002 Feb 1;21(3):418-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11823434 11823434]
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[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Rodriguez, A C.]]
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[[Category: Stock, D.]]
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[[Category: Archaea]]
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[[Category: Dna supercoiling]]
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[[Category: Helicase]]
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[[Category: Topoisomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:42:23 2008''
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Current revision

Reverse gyrase from Archaeoglobus fulgidus

PDB ID 1gku

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