7u5e
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==I-F3b Cascade-TniQ partial R-loop complex== | |
| + | <StructureSection load='7u5e' size='340' side='right'caption='[[7u5e]], [[Resolution|resolution]] 4.03Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7u5e]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_salmonicida Aeromonas salmonicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U5E FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.03Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u5e OCA], [https://pdbe.org/7u5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u5e RCSB], [https://www.ebi.ac.uk/pdbsum/7u5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u5e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems. We demonstrate that transposon protein TniQ plays a central role in promoting R-loop formation by RNA-guided DNA-targeting modules. TniQ residues, proximal to CRISPR RNA (crRNA), are required for recognizing different crRNA categories, revealing an unappreciated role of TniQ to direct transposition into different classes of crRNA targets. To investigate adaptations allowing CAST elements to utilize attachment sites inaccessible to CRISPR-Cas surveillance complexes, we compared and contrasted PAM sequence requirements in both I-F3b CAST and I-F1 CRISPR-Cas systems. We identify specific amino acids that enable a wider range of PAM sequences to be accommodated in I-F3b CAST elements compared with I-F1 CRISPR-Cas, enabling CAST elements to access attachment sites as sequences drift and evade host surveillance. Together, this evidence points to the central role of TniQ in facilitating the acquisition of CRISPR effector complexes for RNA-guided DNA transposition. | ||
| - | + | Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition.,Park JU, Petassi MT, Hsieh SC, Mehrotra E, Schuler G, Budhathoki J, Truong VH, Thyme SB, Ke A, Kellogg EH, Peters JE Mol Cell. 2023 Jun 1;83(11):1827-1838.e6. doi: 10.1016/j.molcel.2023.05.005. PMID:37267904<ref>PMID:37267904</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Kellogg | + | <div class="pdbe-citations 7u5e" style="background-color:#fffaf0;"></div> |
| - | [[Category: Mehrotra | + | == References == |
| - | [[Category: Park | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aeromonas salmonicida]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kellogg EH]] | ||
| + | [[Category: Mehrotra E]] | ||
| + | [[Category: Park JU]] | ||
Current revision
I-F3b Cascade-TniQ partial R-loop complex
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