7s5i

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==Crystal structure of Aldose-6-phosphate reductase (Ald6PRase) from peach (Prunus persica) leaves==
==Crystal structure of Aldose-6-phosphate reductase (Ald6PRase) from peach (Prunus persica) leaves==
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<StructureSection load='7s5i' size='340' side='right'caption='[[7s5i]]' scene=''>
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<StructureSection load='7s5i' size='340' side='right'caption='[[7s5i]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5I FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7s5i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Prunus_persica Prunus persica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5I FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5i OCA], [https://pdbe.org/7s5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5i RCSB], [https://www.ebi.ac.uk/pdbsum/7s5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5i ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5i OCA], [https://pdbe.org/7s5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5i RCSB], [https://www.ebi.ac.uk/pdbsum/7s5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5i ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A5JUQ9_PRUPE A5JUQ9_PRUPE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sugar-alcohols are major photosynthetic products in plant species from the Apiaceae and Plantaginaceae families. Mannose-6-phosphate reductase (Man6PRase) and aldose-6-phosphate reductase (Ald6PRase) are key enzymes for synthesizing mannitol and glucitol in celery (Apium graveolens) and peach (Prunus persica), respectively. In this work, we report the first crystal structures of dimeric plant aldo/keto reductases, celery Man6PRase (solved in the presence of mannonic acid and NADP+) and peach Ald6PRase (obtained in the apo-form). Both structures displayed the typical TIM barrel folding commonly observed in proteins from the aldo/keto reductase superfamily. Analysis of the Man6PRase holo-form showed that residues putatively involved in the catalytic mechanism are located close to the nicotinamide ring from NADP+, where the hydride transfer to the sugar-phosphate should take place. Additionally, we found that Lys48 is important for the binding of the sugar-phosphate. Interestingly, the Man6PRase K48A mutant had a lower catalytic efficiency with mannose 6-phosphate but higher catalytic efficiency with mannose than the wild type. Overall, our work sheds light on the structure-function relationships of important enzymes to synthesize sugar-alcohols in plants.
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Structural Determinants of Sugar-Alcohol Biosynthesis in Plants: The Crystal Structures of Mannose-6-Phosphate and Aldose-6-Phosphate Reductases.,Minen RI, Bhayani JA, Hartman MD, Cereijo AE, Zheng Y, Ballicora MA, Iglesias AA, Liu D, Figueroa CM Plant Cell Physiol. 2022 Mar 3. pii: 6542136. doi: 10.1093/pcp/pcac029. PMID:35243499<ref>PMID:35243499</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7s5i" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Prunus persica]]
[[Category: Ballicora MA]]
[[Category: Ballicora MA]]
[[Category: Bhayani JA]]
[[Category: Bhayani JA]]

Current revision

Crystal structure of Aldose-6-phosphate reductase (Ald6PRase) from peach (Prunus persica) leaves

PDB ID 7s5i

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