5ks7

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<StructureSection load='5ks7' size='340' side='right'caption='[[5ks7]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='5ks7' size='340' side='right'caption='[[5ks7]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ks7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KS7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ks7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KS7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2BA:(2R,3R,3AS,5R,7AR,9R,10R,10AS,12R,14AR)-2,9-BIS(6-AMINO-9H-PURIN-9-YL)OCTAHYDRO-2H,7H-DIFURO[3,2-D 3,2-J][1,3,7,9,2,8]TETRAOXADIPHOSPHACYCLODODECINE-3,5,10,12-TETROL+5,12-DIOXIDE'>2BA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5ks8|5ks8]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2BA:(2R,3R,3AS,5R,7AR,9R,10R,10AS,12R,14AR)-2,9-BIS(6-AMINO-9H-PURIN-9-YL)OCTAHYDRO-2H,7H-DIFURO[3,2-D 3,2-J][1,3,7,9,2,8]TETRAOXADIPHOSPHACYCLODODECINE-3,5,10,12-TETROL+5,12-DIOXIDE'>2BA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">opuCA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Quaternary-amine-transporting_ATPase Quaternary-amine-transporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.32 3.6.3.32] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ks7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks7 OCA], [https://pdbe.org/5ks7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ks7 RCSB], [https://www.ebi.ac.uk/pdbsum/5ks7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ks7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks7 OCA], [https://pdbe.org/5ks7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ks7 RCSB], [https://www.ebi.ac.uk/pdbsum/5ks7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks7 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/OPUCA_LISMN OPUCA_LISMN]
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Cellular turgor is of fundamental importance to bacterial growth and survival. Changes in external osmolarity as a consequence of fluctuating environmental conditions and colonization of diverse environments can significantly impact cytoplasmic water content, resulting in cellular lysis or plasmolysis. To ensure maintenance of appropriate cellular turgor, bacteria import ions and small organic osmolytes, deemed compatible solutes, to equilibrate cytoplasmic osmolarity with the extracellular environment. Here, we show that elevated levels of c-di-AMP, a ubiquitous second messenger among bacteria, result in significant susceptibility to elevated osmotic stress in the bacterial pathogen Listeria monocytogenes. We found that levels of import of the compatible solute carnitine show an inverse correlation with intracellular c-di-AMP content and that c-di-AMP directly binds to the CBS domain of the ATPase subunit of the carnitine importer OpuC. Biochemical and structural studies identify conserved residues required for this interaction and transport activity in bacterial cells. Overall, these studies reveal a role for c-di-AMP mediated regulation of compatible solute import and provide new insight into the molecular mechanisms by which this essential second messenger impacts bacterial physiology and adaptation to changing environmental conditions. This article is protected by copyright. All rights reserved.
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Cyclic di-AMP targets the cystathionine beta-synthase domain of the osmolyte transporter OpuC.,Huynh TN, Choi PH, Sureka K, Ledvina HE, Campillo J, Tong L, Woodward JJ Mol Microbiol. 2016 Jul 5. doi: 10.1111/mmi.13456. PMID:27378384<ref>PMID:27378384</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5ks7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacterium monocytogenes hominis nyfeldt 1932]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Quaternary-amine-transporting ATPase]]
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[[Category: Listeria monocytogenes]]
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[[Category: Choi, P H]]
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[[Category: Choi PH]]
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[[Category: Tong, L]]
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[[Category: Tong L]]
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[[Category: Abc transporter]]
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[[Category: Cbs domain]]
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[[Category: Cyclic-di-amp]]
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[[Category: Hydrolase-hydrolase inhibitor complex]]
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[[Category: Osmoregulation]]
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Current revision

Crystal structure of Listeria monocytogenes OpuCA CBS domain dimer in complex with cyclic-di-AMP

PDB ID 5ks7

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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