1gpc

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[[Image:1gpc.gif|left|200px]]
 
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==CORE GP32, DNA-BINDING PROTEIN==
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The line below this paragraph, containing "STRUCTURE_1gpc", creates the "Structure Box" on the page.
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<StructureSection load='1gpc' size='340' side='right'caption='[[1gpc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gpc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1gpc| PDB=1gpc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpc OCA], [https://pdbe.org/1gpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpc RCSB], [https://www.ebi.ac.uk/pdbsum/1gpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_BPT4 SSB_BPT4] Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Promotes also the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125).[HAMAP-Rule:MF_04152]<ref>PMID:11459967</ref> <ref>PMID:14871889</ref> <ref>PMID:15507125</ref> <ref>PMID:9079662</ref> <ref>PMID:9079662</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gpc ConSurf].
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<div style="clear:both"></div>
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'''CORE GP32, DNA-BINDING PROTEIN'''
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==See Also==
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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==Overview==
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<references/>
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The single-stranded DNA (ssDNA) binding protein gp32 from bacteriophage T4 is essential for T4 DNA replication, recombination and repair. In vivo gp32 binds ssDNA as the replication fork advances and stimulates replisome processivity and accuracy by a factor of several hundred. Gp32 binding affects nearly every major aspect of DNA metabolism. Among its important functions are: (1) configuring ssDNA templates for efficient use by the replisome including DNA polymerase; (2) melting out adventitious secondary structures; (3) protecting exposed ssDNA from nucleases; and (4) facilitating homologous recombination by binding ssDNA during strand displacement. We have determined the crystal structure of the gp32 DNA binding domain complexed to ssDNA at 2.2 A resolution. The ssDNA binding cleft comprises regions from three structural subdomains and includes a positively charged surface that runs parallel to a series of hydrophobic pockets formed by clusters of aromatic side chains. Although only weak electron density is seen for the ssDNA, it indicates that the phosphate backbone contacts an electropositive cleft of the protein, placing the bases in contact with the hydrophobic pockets. The DNA mobility implied by the weak electron density may reflect the role of gp32 as a sequence-independent ssDNA chaperone allowing the largely unstructured ssDNA to slide freely through the cleft.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus T4]]
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1GPC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPC OCA].
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[[Category: Large Structures]]
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[[Category: Friedman AM]]
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==Reference==
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[[Category: Konigsberg WH]]
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Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7630406 7630406]
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[[Category: Parsons MR]]
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[[Category: Enterobacteria phage t4]]
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[[Category: Shamoo Y]]
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[[Category: Single protein]]
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[[Category: Steitz TA]]
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[[Category: Friedman, A M.]]
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[[Category: Konigsberg, W H.]]
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[[Category: Parsons, M R.]]
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[[Category: Shamoo, Y.]]
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[[Category: Steitz, T A.]]
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[[Category: Single-stranded dna-binding]]
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[[Category: Ssb]]
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[[Category: Zinc binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:51:14 2008''
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Current revision

CORE GP32, DNA-BINDING PROTEIN

PDB ID 1gpc

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