7uid
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Thyclotides peptide nucleic acid in complex with DNA== | |
| + | <StructureSection load='7uid' size='340' side='right'caption='[[7uid]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7uid]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UID FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NR0:N-[(3R,4R)-4-aminooxolan-3-yl]-N-[(6-amino-9H-purin-9-yl)acetyl]glycine'>NR0</scene>, <scene name='pdbligand=NRL:2-[2-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)ethanoyl-[(3~{R},4~{R})-4-azanyloxolan-3-yl]amino]ethanoic+acid'>NRL</scene>, <scene name='pdbligand=NSF:N-[(3R,4R)-4-aminooxolan-3-yl]-N-[(5-iodo-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetyl]glycine'>NSF</scene>, <scene name='pdbligand=NSU:2-[[(3~{R},4~{R})-4-azanyloxolan-3-yl]-[2-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]ethanoyl]amino]ethanoic+acid'>NSU</scene>, <scene name='pdbligand=XDV:2-[2-(2-aminoethoxy)ethoxy]acetamide'>XDV</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uid OCA], [https://pdbe.org/7uid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uid RCSB], [https://www.ebi.ac.uk/pdbsum/7uid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uid ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Selective incorporation of conformational constraints into thyclotides can be used to modulate their binding to complementary oligonucleotides, increase polarity, and optimize uptake into HCT116 cells without assistance from moieties known to promote cell uptake. The X-ray structure and biophysical studies of a thyclotide-DNA duplex reveal that incorporation of tetrahydrofurans into an aegPNA backbone promotes a helical conformation that enhances binding to complementary DNA and RNA. Selective incorporation of tetrahydrofurans into the aegPNA backbone allows polarity to be increased incrementally so that uptake into HCT116 cells can be optimized. The enhanced binding, polarity, and cellular uptake properties of thyclotides were used to demonstrate effective inhibition of microRNA-21 in HCT116 cells. | ||
| - | + | Variation of Tetrahydrofurans in Thyclotides Enhances Oligonucleotide Binding and Cellular Uptake of Peptide Nucleic Acids.,Zheng H, Clausse V, Amarasekara H, Mazur SJ, Botos I, Appella DH JACS Au. 2023 Jun 29;3(7):1952-1964. doi: 10.1021/jacsau.3c00198. eCollection , 2023 Jul 24. PMID:37502163<ref>PMID:37502163</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7uid" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Appella DH]] | ||
| + | [[Category: Botos I]] | ||
Current revision
Thyclotides peptide nucleic acid in complex with DNA
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