1gsh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:26, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1gsh.gif|left|200px]]
 
-
<!--
+
==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==
-
The line below this paragraph, containing "STRUCTURE_1gsh", creates the "Structure Box" on the page.
+
<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_B Escherichia coli B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [https://pdbe.org/1gsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [https://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>
-
{{STRUCTURE_1gsh| PDB=1gsh | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GSHB_ECOLI GSHB_ECOLI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsh_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsh ConSurf].
 +
<div style="clear:both"></div>
-
'''STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5'''
+
==See Also==
-
 
+
*[[Glutathione synthetase|Glutathione synthetase]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The crystal structure of Escherichia coli B glutathione synthetase (GSHase) has been determined at the optimal catalytic condition pH 7.5. The most significant structural difference from the structure at pH 6.0 is the movement of the central domain towards the N-terminal domain almost as a rigid body. As a result of this movement, new interdomain and intersubunit polar interactions are formed which stabilize the dimeric structure further. The structure of GSHase at optimal pH was compared with 294 other known protein structures in terms of the spatial arrangements of secondary structural elements. Three enzymes (D-alanine: D-alanine ligase, succinyl-CoA synthetase and the biotin carboxylase subunit of acetyl-CoA carboxylase) were found to have structures similar to the ATP-binding site of GSHase, which extends across two domains. The ATP-binding sites in these four enzymes are composed of two antiparallel beta-sheets and are different from the classic mononucleotide-binding fold. Except for these proteins, no significant structural similarity was detected between GSHase and the other ATP-binding proteins. A structural motif in the N-terminal domain of GSHase has been found to be similar to the NAD-binding fold. This structural motif is shared by a number of other proteins that bind various negatively charged molecules.
+
[[Category: Escherichia coli B]]
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: Kato H]]
-
1GSH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA].
+
[[Category: Katsube Y]]
-
 
+
[[Category: Matsuda K]]
-
==Reference==
+
[[Category: Nishioka T]]
-
Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins., Matsuda K, Mizuguchi K, Nishioka T, Kato H, Go N, Oda J, Protein Eng. 1996 Dec;9(12):1083-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9010922 9010922]
+
[[Category: Oda J]]
-
[[Category: Escherichia coli]]
+
[[Category: Yamaguchi H]]
-
[[Category: Glutathione synthase]]
+
-
[[Category: Single protein]]
+
-
[[Category: Kato, H.]]
+
-
[[Category: Katsube, Y.]]
+
-
[[Category: Matsuda, K.]]
+
-
[[Category: Nishioka, T.]]
+
-
[[Category: Oda, J.]]
+
-
[[Category: Yamaguchi, H.]]
+
-
[[Category: Glutathione biosynthesis]]
+
-
[[Category: Glutathione synthase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:57:14 2008''
+

Current revision

STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5

PDB ID 1gsh

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gsh"
Personal tools