|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==solution structure of apo-NmtR== | | ==solution structure of apo-NmtR== |
| - | <StructureSection load='2lkp' size='340' side='right'caption='[[2lkp]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> | + | <StructureSection load='2lkp' size='340' side='right'caption='[[2lkp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lkp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lkp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKP FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nmtR, Rv3744 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkp OCA], [https://pdbe.org/2lkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkp RCSB], [https://www.ebi.ac.uk/pdbsum/2lkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkp ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkp OCA], [https://pdbe.org/2lkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkp RCSB], [https://www.ebi.ac.uk/pdbsum/2lkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/NMTR_MYCTU NMTR_MYCTU]] Represses transcription of ctpJ/nmtA, by binding to its promoter region.<ref>PMID:12163508</ref>
| + | [https://www.uniprot.org/uniprot/Q7D4Y3_MYCTO Q7D4Y3_MYCTO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Giedroc, D]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Lee, C]] | + | [[Category: Giedroc D]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Lee C]] |
| - | [[Category: Symmetric homodimer]]
| + | |
| - | [[Category: Transcription regulator]]
| + | |
| Structural highlights
Function
Q7D4Y3_MYCTO
Publication Abstract from PubMed
Mycobacterium tuberculosis is an obligate human respiratory pathogen that encodes approximately 10 arsenic repressor (ArsR) family regulatory proteins that allow the organism to respond to a wide range of changes in its immediate microenvironment. How individual ArsR repressors have evolved to respond to selective stimuli is of intrinsic interest. The Ni(II)/Co(II)-specific repressor NmtR and related actinomycete nickel sensors harbor a conserved N-terminal alpha-NH(2)-Gly2-His3-Gly4 sequence. Here, we present the solution structure of homodimeric apo-NmtR and show that the core of the molecule adopts a typical winged-helix ArsR repressor (alpha1-alpha2-alpha3-alphaR-beta1-beta2-alpha5) "open conformation" that is similar to that of the related zinc sensor Staphylococcus aureus CzrA, but harboring long, flexible N-terminal (residues 2-16) and C-terminal (residues 109-120) extensions. Binding of Ni(II) to the regulatory sites induces strong paramagnetic broadening of the alpha5 helical region and the extreme N-terminal tail to residue 10. Ratiometric pulse chase amidination mass spectrometry reveals that the rate of amidination of the alpha-amino group of Gly2 is strongly attenuated in the Ni(II) complex relative to the apo state and noncognate Zn(II) complex. Ni(II) binding also induces dynamic disorder on the microsecond to millisecond time scale of key DNA interacting regions that likely contributes to the negative regulation of DNA binding by Ni(II). Molecular dynamics simulations and quantum chemical calculations reveal that NmtR readily accommodates a distal Ni(II) hexacoordination model involving the alpha-amine and His3 of the N-terminal region and alpha5 residues Asp91', His93', His104, and His107, which collectively define a new metal sensing site configuration in ArsR family regulators.
Solution Structure of Mycobacterium tuberculosis NmtR in the Apo State: Insights into Ni(II)-Mediated Allostery.,Lee CW, Chakravorty DK, Chang FM, Reyes-Caballero H, Ye Y, Merz KM Jr, Giedroc DP Biochemistry. 2012 Mar 27;51(12):2619-29. Epub 2012 Mar 14. PMID:22394357[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee CW, Chakravorty DK, Chang FM, Reyes-Caballero H, Ye Y, Merz KM Jr, Giedroc DP. Solution Structure of Mycobacterium tuberculosis NmtR in the Apo State: Insights into Ni(II)-Mediated Allostery. Biochemistry. 2012 Mar 27;51(12):2619-29. Epub 2012 Mar 14. PMID:22394357 doi:10.1021/bi3001402
|
