7o05
From Proteopedia
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==Crystal structure of SARS-CoV-2 N-CTD== | ==Crystal structure of SARS-CoV-2 N-CTD== | ||
| - | <StructureSection load='7o05' size='340' side='right'caption='[[7o05]]' scene=''> | + | <StructureSection load='7o05' size='340' side='right'caption='[[7o05]], [[Resolution|resolution]] 1.94Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O05 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o05]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O05 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o05 OCA], [https://pdbe.org/7o05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o05 RCSB], [https://www.ebi.ac.uk/pdbsum/7o05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o05 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o05 OCA], [https://pdbe.org/7o05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o05 RCSB], [https://www.ebi.ac.uk/pdbsum/7o05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o05 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The SARS-CoV-2 nucleocapsid protein (N) is responsible for RNA binding. Here we report the crystal structure of the C-terminal domain (N(CTD)) in open and closed conformations and in complex with guanine triphosphate, GTP. The crystal structure and biochemical studies reveal a specific interaction between the guanine, a nucleotide enriched in the packaging signals regions of coronaviruses, and a highly conserved tryptophan residue (W330). In addition, EMSA assays with SARS-CoV-2 derived RNA hairpin loops from a putative viral packaging sequence showed the preference interaction of the N-CTD to RNA oligonucleotides containing G and the loss of the specificity in the mutant W330A. Here we propose that this interaction may facilitate the viral assembly process. In summary, we have identified a specific guanine-binding pocket in the N protein that may be used to design viral assembly inhibitors. | ||
| + | |||
| + | Identification of a guanine-specific pocket in the protein N of SARS-CoV-2.,Rafael Ciges-Tomas J, Franco ML, Vilar M Commun Biol. 2022 Jul 16;5(1):711. doi: 10.1038/s42003-022-03647-8. PMID:35842466<ref>PMID:35842466</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7o05" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Severe acute respiratory syndrome coronavirus 2]] | ||
[[Category: Ciges-Tomas JR]] | [[Category: Ciges-Tomas JR]] | ||
[[Category: Vilar M]] | [[Category: Vilar M]] | ||
Current revision
Crystal structure of SARS-CoV-2 N-CTD
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