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| - | [[Image:1gwl.jpg|left|200px]] | |
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| - | <!--
| + | ==Carbohydrate binding module family29 complexed with mannohexaose== |
| - | The line below this paragraph, containing "STRUCTURE_1gwl", creates the "Structure Box" on the page.
| + | <StructureSection load='1gwl' size='340' side='right'caption='[[1gwl]], [[Resolution|resolution]] 1.51Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1gwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_sp._'equi' Piromyces sp. 'equi']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWL FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> |
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| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr> |
| - | {{STRUCTURE_1gwl| PDB=1gwl | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwl OCA], [https://pdbe.org/1gwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwl RCSB], [https://www.ebi.ac.uk/pdbsum/1gwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwl ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE''' | + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/Q9C171_PIREQ Q9C171_PIREQ] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | Carbohydrate-protein recognition is central to many biological processes. Enzymes that act on polysaccharide substrates frequently contain noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target the enzyme to the appropriate substrate. CBMs that recognize specific plant structural polysaccharides are often able to accommodate both the variable backbone and the side-chain decorations of heterogeneous ligands. "CBM29" modules, derived from a noncatalytic component of the Piromyces equi cellulase/hemicellulase complex, provide an example of this selective yet flexible recognition. They discriminate strongly against some polysaccharides while remaining relatively promiscuous toward both beta-1,4-linked manno- and cello-oligosaccharides. This feature may reflect preferential, but flexible, targeting toward glucomannans in the plant cell wall. The three-dimensional structure of CBM29-2 and its complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of the aromatic residues complements the conformation of the target sugar polymer while accommodation of both manno- and gluco-configured oligo- and polysaccharides is conferred by virtue of the plasticity of the direct interactions from their axial and equatorial 2-hydroxyls, respectively. Such flexible ligand recognition targets the anaerobic fungal complex to a range of different components in the plant cell wall and thus plays a pivotal role in the highly efficient degradation of this composite structure by the microbial eukaryote.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Piromyces sp. 'equi']] |
| - | ==About this Structure== | + | [[Category: Charnock SJ]] |
| - | 1GWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWL OCA].
| + | [[Category: Davies GJ]] |
| - | | + | [[Category: Nurizzo D]] |
| - | ==Reference== | + | |
| - | Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12391332 12391332]
| + | |
| - | [[Category: Piromyces equi]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Charnock, S J.]] | + | |
| - | [[Category: Davies, G J.]] | + | |
| - | [[Category: Nurizzo, D.]] | + | |
| - | [[Category: Carbohydrate binding domain]] | + | |
| - | [[Category: Cellohexaose]] | + | |
| - | [[Category: Cellulosome]] | + | |
| - | [[Category: Glucomannan]] | + | |
| - | [[Category: Mannohexaose]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:06:11 2008''
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