3it5

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of the LasA Virulence Factor from Pseudomonas aeruginosa

Structural highlights

3it5 is a 4 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LASA_PSEAE Involved in proteolysis and elastolysis. Degradation of elastin is likely to contribute to the pathogenicity of P.aeruginosa.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Pseudomonas aeruginosa is an opportunist Gram-negative bacterial pathogen responsible for a wide range of infections in immunocompromized individuals and is a leading cause of mortality in cystic fibrosis patients. A number of secreted virulence factors, including various proteolytic enzymes, contribute to the establishment and maintenance of Pseudomonas infection. One such is LasA, an M23 metallopeptidase related to autolytic glycylglycine endopeptidases such as Staphylococcus aureus lysostaphin and LytM, and to DD-endopeptidases involved in entry of bacteriophage to host bacteria. LasA is implicated in a range of processes related to Pseudomonas virulence, including stimulating ectodomain shedding of the cell surface heparan sulphate proteoglycan syndecan-1 and elastin degradation in connective tissue. Here we present crystal structures of active LasA as a complex with tartrate and in the uncomplexed form. While the overall fold resembles that of the other M23 family members, the LasA active site is less constricted and utilizes a different set of metal ligands. The active site of uncomplexed LasA contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules. Using these structures as a starting point, we propose a model for substrate binding by LasA that explains its activity against a wider range of substrates than those used by related lytic enzymes, and offer a catalytic mechanism for M23 metallopeptidases consistent with available structural and mutagenesis data. Our results highlight how LasA is a structurally distinct member of this endopeptidase family, consistent with its activity against a wider range of substrates and with its multiple roles in Pseudomonas virulence.

Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases.,Spencer J, Murphy LM, Conners R, Sessions RB, Gamblin SJ J Mol Biol. 2010 Mar 5;396(4):908-23. Epub 2009 Dec 21. PMID:20026068^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spencer J, Murphy LM, Conners R, Sessions RB, Gamblin SJ. Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases. J Mol Biol. 2010 Mar 5;396(4):908-23. Epub 2009 Dec 21. PMID:20026068 doi:10.1016/j.jmb.2009.12.021

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3it5"

@@ Line 3: / Line 3: @@
 <StructureSection load='3it5' size='340' side='right'caption='[[3it5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3it5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IT5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3it5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IT5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3it7|3it7]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3it5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3it5 OCA], [https://pdbe.org/3it5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3it5 RCSB], [https://www.ebi.ac.uk/pdbsum/3it5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3it5 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LASA_PSEAE LASA_PSEAE]] Involved in proteolysis and elastolysis. Degradation of elastin is likely to contribute to the pathogenicity of P.aeruginosa.
+[https://www.uniprot.org/uniprot/LASA_PSEAE LASA_PSEAE] Involved in proteolysis and elastolysis. Degradation of elastin is likely to contribute to the pathogenicity of P.aeruginosa.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/3it5_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 34: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pseudomonas aeruginosa]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Conners, R]]
+[[Category: Conners R]]
-[[Category: Gamblin, S J]]
+[[Category: Gamblin SJ]]
-[[Category: Murphy, L M]]
+[[Category: Murphy LM]]
-[[Category: Sessions, R B]]
+[[Category: Sessions RB]]
-[[Category: Spencer, J]]
+[[Category: Spencer J]]
-[[Category: Beta-protein]]
-[[Category: Cell membrane]]
-[[Category: Cell outer membrane]]
-[[Category: Hydrolase]]
-[[Category: M23]]
-[[Category: Membrane]]
-[[Category: Metal-binding]]
-[[Category: Metallopeptidase]]
-[[Category: Metalloprotease]]
-[[Category: Protease]]
-[[Category: Zymogen]]

3it5

From Proteopedia

Current revision

Crystal Structure of the LasA Virulence Factor from Pseudomonas aeruginosa

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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