3j4u

<table><tr><td colspan='2'>[[3j4u]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_phage_bpp-1 Bordetella phage bpp-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J4U FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4u OCA], [https://pdbe.org/3j4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j4u RCSB], [https://www.ebi.ac.uk/pdbsum/3j4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4u ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures. Here, we report a cryo electron microscopy structure of the BPP-1 head at 3.5 A resolution. Our atomic model shows two of the three protein folds representing major viral lineages: jellyroll for its cement protein (CP) and HK97-like ('Johnson') for its major capsid protein (MCP). Strikingly, the fold topology of MCP is permuted non-circularly from the Johnson fold topology previously seen in viral and cellular proteins. We illustrate that the new topology is likely the only feasible alternative of the old topology. beta-sheet augmentation and electrostatic interactions contribute to the formation of non-covalent chainmail in BPP-1, unlike covalent inter-protein linkages of the HK97 chainmail. Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering. DOI: http://dx.doi.org/10.7554/eLife.01299.001.

 

 

<references/>

[[Category: Bordetella phage bpp-1]]

[[Category: Czornyj, E]]

[[Category: Guo, H]]

[[Category: Hodes, A]]

[[Category: Hui, W H]]

[[Category: Jin, L]]

[[Category: Miller, J F]]

[[Category: Nieh, A W]]

[[Category: Zhang, X]]

[[Category: Zhou, Z H]]

[[Category: Virus]]