2yc4

From Proteopedia

(Difference between revisions)

Current revision

Intraflagellar Transport Complex 25-27 from Chlamydomonas

Structural highlights

2yc4 is a 4 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IFT25_CHLRE Component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT27.^[1]

Publication Abstract from PubMed

The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 A resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex.

Crystal structure of the intraflagellar transport complex 25/27.,Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E EMBO J. 2011 Apr 19. PMID:21505417^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang Z, Fan ZC, Williamson SM, Qin H. Intraflagellar transport (IFT) protein IFT25 is a phosphoprotein component of IFT complex B and physically interacts with IFT27 in Chlamydomonas. PLoS One. 2009;4(5):e5384. PMID:19412537 doi:10.1371/journal.pone.0005384
↑ Bhogaraju S, Taschner M, Morawetz M, Basquin C, Lorentzen E. Crystal structure of the intraflagellar transport complex 25/27. EMBO J. 2011 Apr 19. PMID:21505417 doi:10.1038/emboj.2011.110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yc4"

Categories: Chlamydomonas reinhardtii | Large Structures | Bhogaraju S | Lorentzen E | Taschner M

@@ Line 3: / Line 3: @@
 <StructureSection load='2yc4' size='340' side='right'caption='[[2yc4]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2yc4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YC4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2yc4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YC4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2yc2|2yc2]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc4 OCA], [https://pdbe.org/2yc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yc4 RCSB], [https://www.ebi.ac.uk/pdbsum/2yc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yc4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/IFT27_CHLRE IFT27_CHLRE]] Small GTPase-like component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT25. Has very low GTPase activity either because it lacks the conserved catalytic Gln in position 79 or because it requires some GTPase-activating protein (GAP) for GTP turnover.<ref>PMID:21505417</ref>
+[https://www.uniprot.org/uniprot/IFT25_CHLRE IFT25_CHLRE] Component of the intraflagellar transport (IFT) complex B. Forms a subcomplex within the IFT complex B with IFT27.<ref>PMID:19412537</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Chlre]]
+[[Category: Chlamydomonas reinhardtii]]
 [[Category: Large Structures]]
-[[Category: Bhogaraju, S]]
+[[Category: Bhogaraju S]]
-[[Category: Lorentzen, E]]
+[[Category: Lorentzen E]]
-[[Category: Taschner, M]]
+[[Category: Taschner M]]
-[[Category: Cilium]]
-[[Category: Ift complex]]
-[[Category: Transport protein]]

2yc4

From Proteopedia

Current revision

Intraflagellar Transport Complex 25-27 from Chlamydomonas

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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