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2ykp

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Structure of the human LINE-1 ORF1p trimer

Structural highlights

2ykp is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LORF1_HUMAN Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.^[1] ^[2] ^[3]

Publication Abstract from PubMed

The LINE-1 (L1) retrotransposon emerges as a major source of human interindividual genetic variation, with important implications for evolution and disease. L1 retrotransposition is poorly understood at the molecular level, and the mechanistic details and evolutionary origin of the L1-encoded L1ORF1 protein (L1ORF1p) are particularly obscure. Here three crystal structures of trimeric L1ORF1p and NMR solution structures of individual domains reveal a sophisticated and highly structured, yet remarkably flexible, RNA-packaging protein. It trimerizes via an N-terminal, ion-containing coiled coil that serves as scaffold for the flexible attachment of the central RRM and the C-terminal CTD domains. The structures explain the specificity for single-stranded RNA substrates, and a mutational analysis indicates that the precise control of domain flexibility is critical for retrotransposition. Although the evolutionary origin of L1ORF1p remains unclear, our data reveal previously undetected structural and functional parallels to viral proteins.

Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition.,Khazina E, Truffault V, Buttner R, Schmidt S, Coles M, Weichenrieder O Nat Struct Mol Biol. 2011 Aug 7. doi: 10.1038/nsmb.2097. PMID:21822284^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wei W, Gilbert N, Ooi SL, Lawler JF, Ostertag EM, Kazazian HH, Boeke JD, Moran JV. Human L1 retrotransposition: cis preference versus trans complementation. Mol Cell Biol. 2001 Feb;21(4):1429-39. PMID:11158327 doi:http://dx.doi.org/10.1128/MCB.21.4.1429-1439.2001
↑ Callahan KE, Hickman AB, Jones CE, Ghirlando R, Furano AV. Polymerization and nucleic acid-binding properties of human L1 ORF1 protein. Nucleic Acids Res. 2012 Jan;40(2):813-27. doi: 10.1093/nar/gkr728. Epub 2011 Sep , 21. PMID:21937507 doi:http://dx.doi.org/10.1093/nar/gkr728
↑ Moran JV, Holmes SE, Naas TP, DeBerardinis RJ, Boeke JD, Kazazian HH Jr. High frequency retrotransposition in cultured mammalian cells. Cell. 1996 Nov 29;87(5):917-27. PMID:8945518
↑ Khazina E, Truffault V, Buttner R, Schmidt S, Coles M, Weichenrieder O. Trimeric structure and flexibility of the L1ORF1 protein in human L1 retrotransposition. Nat Struct Mol Biol. 2011 Aug 7. doi: 10.1038/nsmb.2097. PMID:21822284 doi:10.1038/nsmb.2097

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ykp"

Categories: Homo sapiens | Large Structures | Khazina E | Weichenrieder O

@@ Line 3: / Line 3: @@
 <StructureSection load='2ykp' size='340' side='right'caption='[[2ykp]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ykp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YKP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ykp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YKP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2w7a|2w7a]], [[2jrb|2jrb]], [[2yko|2yko]], [[2ykq|2ykq]], [[2ldy|2ldy]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ykp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ykp OCA], [https://pdbe.org/2ykp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ykp RCSB], [https://www.ebi.ac.uk/pdbsum/2ykp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ykp ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/LORF1_HUMAN LORF1_HUMAN]] Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.<ref>PMID:11158327</ref> <ref>PMID:21937507</ref> <ref>PMID:8945518</ref>
+[https://www.uniprot.org/uniprot/LORF1_HUMAN LORF1_HUMAN] Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.<ref>PMID:11158327</ref> <ref>PMID:21937507</ref> <ref>PMID:8945518</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Khazina, E]]
+[[Category: Khazina E]]
-[[Category: Weichenrieder, O]]
+[[Category: Weichenrieder O]]
-[[Category: Coiled-coil]]
-[[Category: Genome evolution]]
-[[Category: Nucleic acid chaperone]]
-[[Category: Rna binding protein]]
-[[Category: Rna-binding protein]]
-[[Category: Rnp]]

2ykp

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Current revision

Structure of the human LINE-1 ORF1p trimer

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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