7zc0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==4,6-alpha-glucanotransferase GtfC from Geobacillus 12AMOR1== | |
| + | <StructureSection load='7zc0' size='340' side='right'caption='[[7zc0]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7zc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus Geobacillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc0 OCA], [https://pdbe.org/7zc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | GtfC-type 4,6-alpha-glucanotransferase (alpha-GT) enzymes from Glycoside Hydrolase Family 70 (GH70) are of interest for the modification of starch into low-glycemic index food ingredients. Compared to the related GH70 GtfB-type alpha-GTs, found exclusively in lactic acid bacteria (LAB), GtfCs occur in non-LAB, share low sequence identity, lack circular permutation of the catalytic domain, and feature a single-segment auxiliary domain IV and auxiliary C-terminal domains. Despite these differences, the first crystal structure of a GtfC, GbGtfC-DeltaC from Geobacillus 12AMOR1, and the first one representing a non-permuted GH70 enzyme, reveals high structural similarity in the core domains with most GtfBs, featuring a similar tunneled active site. We propose that GtfC (and related GtfD) enzymes evolved from starch-degrading alpha-amylases from GH13 by acquiring alpha-1,6 transglycosylation capabilities, before the events that resulted in circular permutation of the catalytic domain observed in other GH70 enzymes (glucansucrases, GtfB-type alpha-GTs). AlphaFold modeling and sequence alignments suggest that the GbGtfC structure represents the GtfC subfamily, although it has a so far unique alternating alpha-1,4/alpha-1,6 product specificity, likely determined by residues near acceptor binding subsites +1/+2. | ||
| - | + | Crystal Structure of 4,6-alpha-Glucanotransferase GtfC-DeltaC from Thermophilic Geobacillus 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes.,Pijning T, Te Poele EM, de Leeuw TC, Guskov A, Dijkhuizen L J Agric Food Chem. 2022 Nov 28. doi: 10.1021/acs.jafc.2c06394. PMID:36442227<ref>PMID:36442227</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7zc0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Geobacillus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Dijkhuizen L]] | ||
| + | [[Category: Guskov A]] | ||
| + | [[Category: Pijning T]] | ||
| + | [[Category: Te Poele EM]] | ||
Current revision
4,6-alpha-glucanotransferase GtfC from Geobacillus 12AMOR1
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