7n5m
From Proteopedia
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==PCNA from Thermococcus gammatolerans: crystal III, collection 1, 2.00 A, 1.91 MGy== | ==PCNA from Thermococcus gammatolerans: crystal III, collection 1, 2.00 A, 1.91 MGy== | ||
| - | <StructureSection load='7n5m' size='340' side='right'caption='[[7n5m]]' scene=''> | + | <StructureSection load='7n5m' size='340' side='right'caption='[[7n5m]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7n5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_gammatolerans_EJ3 Thermococcus gammatolerans EJ3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N5M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5m OCA], [https://pdbe.org/7n5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5m RCSB], [https://www.ebi.ac.uk/pdbsum/7n5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5m ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n5m OCA], [https://pdbe.org/7n5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n5m RCSB], [https://www.ebi.ac.uk/pdbsum/7n5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n5m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PCNA_THEGJ PCNA_THEGJ] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Proliferating cell nuclear antigen (PCNA) is an essential protein for cell viability in archaea and eukarya, since it is involved in DNA replication and repair. In order to obtain insights regarding the characteristics that confer radioresistance, the structural study of the PCNA from Thermococcus gammatolerans (PCNATg ) in a gradient of ionizing radiation by X-ray crystallography was carried out, together with a bioinformatic analysis of homotrimeric PCNA structures, their sequences, and their molecular interactions. The results obtained from the datasets and the accumulated radiation dose for the last collection from three crystals revealed moderate and localized damage, since even with the loss of resolution, the electron density map corresponding to the last collection allowed to build the whole structure. Attempting to understand this behavior, multiple sequence alignments, and structural superpositions were performed, revealing that PCNA is a protein with a poorly conserved sequence, but with a highly conserved structure. The PCNATg presented the highest percentage of charged residues, mostly negatively charged, with a proportion of glutamate more than double aspartate, lack of cysteines and tryptophan, besides a high number of salt bridges. The structural study by X-ray crystallography reveals that the PCNATg has the intrinsic ability to resist high levels of ionizing radiation, and the bioinformatic analysis suggests that molecular evolution selected a particular composition of amino acid residues, and their consequent network of synergistic interactions for extreme conditions, as a collateral effect, conferring radioresistance to a protein involved in the chromosomal DNA metabolism of a radioresistant microorganism. | ||
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| + | PCNA from Thermococcus gammatolerans: A protein involved in chromosomal DNA metabolism intrinsically resistant at high levels of ionizing radiation.,Marin-Tovar Y, Serrano-Posada H, Diaz-Vilchis A, Rudino-Pinera E Proteins. 2022 Apr 18. doi: 10.1002/prot.26346. PMID:35435259<ref>PMID:35435259</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n5m" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Thermococcus gammatolerans EJ3]] | ||
[[Category: Marin-Tovar Y]] | [[Category: Marin-Tovar Y]] | ||
[[Category: Rudino-Pinera E]] | [[Category: Rudino-Pinera E]] | ||
Current revision
PCNA from Thermococcus gammatolerans: crystal III, collection 1, 2.00 A, 1.91 MGy
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