This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3mlo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:48, 7 February 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='3mlo' size='340' side='right'caption='[[3mlo]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
<StructureSection load='3mlo' size='340' side='right'caption='[[3mlo]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3mlo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MLO FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3mlo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MLO FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3mln|3mln]], [[3mlp|3mlp]]</div></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ebf1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mlo OCA], [https://pdbe.org/3mlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mlo RCSB], [https://www.ebi.ac.uk/pdbsum/3mlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mlo ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mlo OCA], [https://pdbe.org/3mlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mlo RCSB], [https://www.ebi.ac.uk/pdbsum/3mlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mlo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/COE1_MOUSE COE1_MOUSE]] Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.<ref>PMID:20876732</ref>
+
[https://www.uniprot.org/uniprot/COE1_MOUSE COE1_MOUSE] Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.<ref>PMID:20876732</ref>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Early B-cell factor 1 (Ebf1) is a key transcriptional determinant of B-lymphocyte differentiation whose DNA-binding domain has no sequence similarity to other transcription factor families. Here we report the crystal structure of an Ebf1 dimer bound to its palindromic recognition site. The DNA-binding domain adopts a pseudoimmunoglobulin-like fold with novel topology, but is structurally similar to the Rel homology domains of NFAT and NF-kappaB. Ebf1 contacts the DNA with two loop-based modules and a unique Zn coordination motif whereby each Ebf1 monomer interacts with both palindromic half-sites. This unusual mode of DNA recognition generates an extended contact area that may be crucial for the function of Ebf1 in chromatin.
+
-
 
+
-
Structure of an Ebf1:DNA complex reveals unusual DNA recognition and structural homology with Rel proteins.,Treiber N, Treiber T, Zocher G, Grosschedl R Genes Dev. 2010 Oct 15;24(20):2270-5. Epub 2010 Sep 28. PMID:20876732<ref>PMID:20876732</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3mlo" style="background-color:#fffaf0;"></div>
+
== References ==
== References ==
<references/>
<references/>
Line 25: Line 15:
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Lk3 transgenic mice]]
+
[[Category: Mus musculus]]
-
[[Category: Grosschedl, R]]
+
[[Category: Synthetic construct]]
-
[[Category: Treiber, N]]
+
[[Category: Grosschedl R]]
-
[[Category: Treiber, T]]
+
[[Category: Treiber N]]
-
[[Category: Zocher, G]]
+
[[Category: Treiber T]]
-
[[Category: Dna]]
+
[[Category: Zocher G]]
-
[[Category: Ebf]]
+
-
[[Category: Ebf-1]]
+
-
[[Category: Pseudo-ig-fold]]
+
-
[[Category: Transcription factor]]
+
-
[[Category: Transcription-dna complex]]
+
-
[[Category: Zn-finger]]
+
-
[[Category: Zn-knuckle]]
+

Current revision

DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (Crystal form I)

PDB ID 3mlo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mlo"
Personal tools