1exz

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(New page: 200px<br /> <applet load="1exz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1exz, resolution 2.3&Aring;" /> '''STRUCTURE OF STEM CE...)
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[[Image:1exz.gif|left|200px]]<br />
 
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<applet load="1exz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1exz, resolution 2.3&Aring;" />
 
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'''STRUCTURE OF STEM CELL FACTOR'''<br />
 
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==Overview==
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==STRUCTURE OF STEM CELL FACTOR==
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Stem cell factor (SCF) plays important roles in hematopoiesis and the, survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF mediates its biological effects by binding to and, activating a receptor tyrosine kinase designated c-kit or SCF receptor. In, this report we describe the 2.3-A crystal structure of the functional core, of recombinant human SCF. SCF is a noncovalent homodimer composed of two, slightly wedged protomers. Each SCF protomer exhibits an antiparallel, four-helix bundle fold. Dimerization is mediated by extensive polar and, nonpolar interactions between the two protomers with a large buried, surface area. Finally, we have identified a hydrophobic crevice and a, charged region at the tail of each protomer that functions as a potential, receptor-binding site. On the basis of these observations, a model for SCF, small middle dotc-kit complex formation and dimerization is proposed.
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<StructureSection load='1exz' size='340' side='right'caption='[[1exz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1exz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EXZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SM:SAMARIUM+(III)+ION'>SM</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1exz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1exz OCA], [https://pdbe.org/1exz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1exz RCSB], [https://www.ebi.ac.uk/pdbsum/1exz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1exz ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/SCF_HUMAN SCF_HUMAN] Defects in KITLG are the cause of familial progressive hyperpigmentation (FPH) [MIM:[https://omim.org/entry/145250 145250]; also called melanosis universalis hereditaria (MUH). FPH is an autosomal-dominantly inherited disorder characterized by hyperpigmented patches in the skin, present in early infancy and increasing in size and number with age.<ref>PMID:19375057</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/SCF_HUMAN SCF_HUMAN] Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1exz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1exz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Stem cell factor (SCF) plays important roles in hematopoiesis and the survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF mediates its biological effects by binding to and activating a receptor tyrosine kinase designated c-kit or SCF receptor. In this report we describe the 2.3-A crystal structure of the functional core of recombinant human SCF. SCF is a noncovalent homodimer composed of two slightly wedged protomers. Each SCF protomer exhibits an antiparallel four-helix bundle fold. Dimerization is mediated by extensive polar and nonpolar interactions between the two protomers with a large buried surface area. Finally, we have identified a hydrophobic crevice and a charged region at the tail of each protomer that functions as a potential receptor-binding site. On the basis of these observations, a model for SCF small middle dotc-kit complex formation and dimerization is proposed.
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==About this Structure==
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Crystal structure of human stem cell factor: implication for stem cell factor receptor dimerization and activation.,Zhang Z, Zhang R, Joachimiak A, Schlessinger J, Kong XP Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7732-7. PMID:10884405<ref>PMID:10884405</ref>
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1EXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SM, CA and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EXZ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of human stem cell factor: implication for stem cell factor receptor dimerization and activation., Zhang Z, Zhang R, Joachimiak A, Schlessinger J, Kong XP, Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7732-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10884405 10884405]
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</div>
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<div class="pdbe-citations 1exz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Kong, X.]]
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[[Category: Kong X]]
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[[Category: Schlessinger, J.]]
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[[Category: Schlessinger J]]
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[[Category: Zhang, R.]]
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[[Category: Zhang R]]
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[[Category: Zhang, Z.]]
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[[Category: Zhang Z]]
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[[Category: CA]]
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[[Category: SM]]
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[[Category: TRS]]
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[[Category: scf]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:47:38 2007''
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STRUCTURE OF STEM CELL FACTOR

PDB ID 1exz

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