7w7g
From Proteopedia
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| - | ==== | + | ==Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex== |
| - | <StructureSection load='7w7g' size='340' side='right'caption='[[7w7g]]' scene=''> | + | <StructureSection load='7w7g' size='340' side='right'caption='[[7w7g]], [[Resolution|resolution]] 3.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7w7g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W7G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7g OCA], [https://pdbe.org/7w7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7g RCSB], [https://www.ebi.ac.uk/pdbsum/7w7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7g ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w7g OCA], [https://pdbe.org/7w7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w7g RCSB], [https://www.ebi.ac.uk/pdbsum/7w7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w7g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E5CYJ9_MOUSE E5CYJ9_MOUSE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NALCN channel mediates sodium leak currents and is important for maintaining proper resting membrane potential. NALCN and FAM155A form the core complex of the channel, the activity of which essentially depends on the presence of both UNC79 and UNC80, two auxiliary proteins. NALCN, FAM155A, UNC79, and UNC80 co-assemble into a large hetero-tetrameric channel complex. Genetic mutations of NALCN channel components lead to neurodevelopmental diseases. However, the structure and mechanism of the intact channel complex remain elusive. Here, we present the cryo-EM structure of the mammalian NALCN-FAM155A-UNC79-UNC80 quaternary complex. The structure shows that UNC79-UNC80 form a large piler-shaped heterodimer which was tethered to the intracellular side of the NALCN channel through tripartite interactions with the cytoplasmic loops of NALCN. Two interactions are essential for proper cell surface localization of NALCN. The other interaction relieves the self-inhibition of NALCN by pulling the auto-inhibitory CTD Interacting Helix (CIH) out of its binding site. Our work defines the structural mechanism of NALCN modulation by UNC79 and UNC80. | ||
| + | |||
| + | Structure and mechanism of NALCN-FAM155A-UNC79-UNC80 channel complex.,Kang Y, Chen L Nat Commun. 2022 May 12;13(1):2639. doi: 10.1038/s41467-022-30403-7. PMID:35550517<ref>PMID:35550517</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7w7g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| + | [[Category: Rattus norvegicus]] | ||
| + | [[Category: Chen L]] | ||
| + | [[Category: Kang Y]] | ||
Current revision
Structure of Mammalian NALCN-FAM155A-UNC79-UNC80 quanternary complex
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