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2mxq

From Proteopedia

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The solution structure of DEFA1, a highly potent antimicrobial peptide from the horse

Structural highlights

2mxq is a 1 chain structure with sequence from Equus caballus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DEFA1_HORSE Has broad-spectrum antimicrobial properties (PubMed:17620056, PubMed:19211153). The antimicrobial activity decreases in the present of salt in vitro (PubMed:19211153). Binds anionic phospholipids, which leads to the aggregation of liposomes in vitro (PubMed:25769951). Membrane permeabilization of the target cells is an essential part of the peptide's mode of antimicrobial activity (PubMed:17620056, PubMed:22232283). No hemolytic activity against sheep or horse erythrocytes (PubMed:22232283). Has antibacterial activity against the bacterial horse pathogens Gram-positive R.equi ATCC 33701 P(-) (minimum bactericidal concentration or MBC=5 ug/ml) and R.equi ATCC 33701 P(+) (MBC=5 ug/ml), which are resistant against beta-lactam antibiotics. Also has antibacterial activity against highly infectious wild-type strain R.equi 85F P(+) (MBC=5 ug/ml), S.equi subsp. equi (MBC=5 ug/ml), S.equi subsp. zooepidemicus (MBC=5 ug/ml), S.dysgalactiae subsp. equisimilis (MBC=10 ug/ml), S.choleraesuis subsp. choleraesuis serovar Typhimurium (MBC=10 ug/ml), and P.multocida subsp. multocida (MBC=>10 ug/ml) (PubMed:19211153). Probably contributes to the antimicrobial barrier function of the small bowel mucosa (Probable).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Defensins are small effector molecules of the innate immune system that are present in almost all organisms including plants and animals. These peptides possess antimicrobial activity against a broad range of microbes including bacteria, fungi and viruses and act as endogenous antibiotics. alpha-Defensins are a subfamily of the defensin family and their expression is limited to specific tissues. Equine DEFA1 is an enteric alpha-defensin exclusively secreted by Paneth cells and shows an activity against a broad spectrum of microbes, including typical pathogens of the horse such as Rhodococcus equi, various streptococci strains, Salmonella choleraesuis, and Pasteurella multocida. Here, we report the three-dimensional structure of DEFA1 solved by NMR-spectroscopy and demonstrate its specific function of aggregating various phospholipids.

Solution structure and functional studies of the highly potent equine antimicrobial peptide DEFA1.,Michalek M, Jung S, Shomali MR, Cauchard S, Sonnichsen FD, Grotzinger J Biochem Biophys Res Commun. 2015 Apr 17;459(4):668-72. doi:, 10.1016/j.bbrc.2015.02.168. Epub 2015 Mar 11. PMID:25769951^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bruhn O, Regenhard P, Michalek M, Paul S, Gelhaus C, Jung S, Thaller G, Podschun R, Leippe M, Grötzinger J, Kalm E. A novel horse alpha-defensin: gene transcription, recombinant expression and characterization of the structure and function. Biochem J. 2007 Oct 15;407(2):267-76. PMID:17620056 doi:10.1042/BJ20070747
↑ Bruhn O, Cauchard J, Schlusselhuber M, Gelhaus C, Podschun R, Thaller G, Laugier C, Leippe M, Grötzinger J. Antimicrobial properties of the equine alpha-defensin DEFA1 against bacterial horse pathogens. Vet Immunol Immunopathol. 2009 Jul 15;130(1-2):102-6. PMID:19211153 doi:10.1016/j.vetimm.2009.01.005
↑ Schlusselhuber M, Jung S, Bruhn O, Goux D, Leippe M, Leclercq R, Laugier C, Grötzinger J, Cauchard J. In vitro potential of equine DEFA1 and eCATH1 as alternative antimicrobial drugs in rhodococcosis treatment. Antimicrob Agents Chemother. 2012 Apr;56(4):1749-55. PMID:22232283 doi:10.1128/AAC.05797-11
↑ Michalek M, Jung S, Shomali MR, Cauchard S, Sonnichsen FD, Grotzinger J. Solution structure and functional studies of the highly potent equine antimicrobial peptide DEFA1. Biochem Biophys Res Commun. 2015 Apr 17;459(4):668-72. doi:, 10.1016/j.bbrc.2015.02.168. Epub 2015 Mar 11. PMID:25769951 doi:http://dx.doi.org/10.1016/j.bbrc.2015.02.168
↑ Michalek M, Jung S, Shomali MR, Cauchard S, Sonnichsen FD, Grotzinger J. Solution structure and functional studies of the highly potent equine antimicrobial peptide DEFA1. Biochem Biophys Res Commun. 2015 Apr 17;459(4):668-72. doi:, 10.1016/j.bbrc.2015.02.168. Epub 2015 Mar 11. PMID:25769951 doi:http://dx.doi.org/10.1016/j.bbrc.2015.02.168

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mxq"

@@ Line 1: / Line 1: @@
 ==The solution structure of DEFA1, a highly potent antimicrobial peptide from the horse==
-<StructureSection load='2mxq' size='340' side='right'caption='[[2mxq]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2mxq' size='340' side='right'caption='[[2mxq]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mxq]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MXQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mxq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MXQ FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mxq OCA], [https://pdbe.org/2mxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mxq RCSB], [https://www.ebi.ac.uk/pdbsum/2mxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mxq ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/DEFA1_HORSE DEFA1_HORSE] Has broad-spectrum antimicrobial properties (PubMed:17620056, PubMed:19211153). The antimicrobial activity decreases in the present of salt in vitro (PubMed:19211153). Binds anionic phospholipids, which leads to the aggregation of liposomes in vitro (PubMed:25769951). Membrane permeabilization of the target cells is an essential part of the peptide's mode of antimicrobial activity (PubMed:17620056, PubMed:22232283). No hemolytic activity against sheep or horse erythrocytes (PubMed:22232283). Has antibacterial activity against the bacterial horse pathogens Gram-positive R.equi ATCC 33701 P(-) (minimum bactericidal concentration or MBC=5 ug/ml) and R.equi ATCC 33701 P(+) (MBC=5 ug/ml), which are resistant against beta-lactam antibiotics. Also has antibacterial activity against highly infectious wild-type strain R.equi 85F P(+) (MBC=5 ug/ml), S.equi subsp. equi (MBC=5 ug/ml), S.equi subsp. zooepidemicus (MBC=5 ug/ml), S.dysgalactiae subsp. equisimilis (MBC=10 ug/ml), S.choleraesuis subsp. choleraesuis serovar Typhimurium (MBC=10 ug/ml), and P.multocida subsp. multocida (MBC=>10 ug/ml) (PubMed:19211153). Probably contributes to the antimicrobial barrier function of the small bowel mucosa (Probable).<ref>PMID:17620056</ref> <ref>PMID:19211153</ref> <ref>PMID:22232283</ref> <ref>PMID:25769951</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Equus caballus]]
 [[Category: Large Structures]]
-[[Category: Jung, S]]
+[[Category: Jung S]]
-[[Category: Michalek, M]]
+[[Category: Michalek M]]
-[[Category: Shomali, M]]
+[[Category: Shomali M]]
-[[Category: Soennichsen, F D]]
+[[Category: Soennichsen FD]]
-[[Category: Antimicrobial protein]]

2mxq

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Current revision

The solution structure of DEFA1, a highly potent antimicrobial peptide from the horse

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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