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3ouo

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Current revision (11:18, 21 February 2024) (edit) (undo)
 
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<StructureSection load='3ouo' size='340' side='right'caption='[[3ouo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='3ouo' size='340' side='right'caption='[[3ouo]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ouo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rvfv Rvfv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ouo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rift_valley_fever_virus_(STRAIN_ZH-548_M12) Rift valley fever virus (STRAIN ZH-548 M12)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ov9|3ov9]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11589 RVFV])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ouo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouo OCA], [https://pdbe.org/3ouo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ouo RCSB], [https://www.ebi.ac.uk/pdbsum/3ouo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouo ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ouo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouo OCA], [https://pdbe.org/3ouo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ouo RCSB], [https://www.ebi.ac.uk/pdbsum/3ouo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouo ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/NCAP_RVFVZ NCAP_RVFVZ]
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Rift Valley fever virus (RVFV), a Phlebovirus with a genome consisting of three single-stranded RNA segments, is spread by infected mosquitoes and causes large viral outbreaks in Africa. RVFV encodes a nucleoprotein (N) that encapsidates the viral RNA. The N protein is the major component of the ribonucleoprotein complex and is also required for genomic RNA replication and transcription by the viral polymerase. Here we present the 1.6 A crystal structure of the RVFV N protein in hexameric form. The ring-shaped hexamers form a functional RNA binding site, as assessed by mutagenesis experiments. Electron microscopy (EM) demonstrates that N in complex with RNA also forms rings in solution, and a single-particle EM reconstruction of a hexameric N-RNA complex is consistent with the crystallographic N hexamers. The ring-like organization of the hexamers in the crystal is stabilized by circular interactions of the N terminus of RVFV N, which forms an extended arm that binds to a hydrophobic pocket in the core domain of an adjacent subunit. The conformation of the N-terminal arm differs from that seen in a previous crystal structure of RVFV, in which it was bound to the hydrophobic pocket in its own core domain. The switch from an intra- to an inter-molecular interaction mode of the N-terminal arm may be a general principle that underlies multimerization and RNA encapsidation by N proteins from Bunyaviridae. Furthermore, slight structural adjustments of the N-terminal arm would allow RVFV N to form smaller or larger ring-shaped oligomers and potentially even a multimer with a super-helical subunit arrangement. Thus, the interaction mode between subunits seen in the crystal structure would allow the formation of filamentous ribonucleocapsids in vivo. Both the RNA binding cleft and the multimerization site of the N protein are promising targets for the development of antiviral drugs.
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The hexamer structure of Rift Valley fever virus nucleoprotein suggests a mechanism for its assembly into ribonucleoprotein complexes.,Ferron F, Li Z, Danek EI, Luo D, Wong Y, Coutard B, Lantez V, Charrel R, Canard B, Walz T, Lescar J PLoS Pathog. 2011 May;7(5):e1002030. doi: 10.1371/journal.ppat.1002030. Epub 2011, May 12. PMID:21589902<ref>PMID:21589902</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ouo" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Rvfv]]
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[[Category: Canard B]]
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[[Category: Canard, B]]
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[[Category: Charrel R]]
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[[Category: Charrel, R]]
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[[Category: Coutard B]]
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[[Category: Coutard, B]]
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[[Category: Danek EI]]
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[[Category: Danek, E I]]
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[[Category: Ferron F]]
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[[Category: Ferron, F]]
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[[Category: Lantez V]]
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[[Category: Lantez, V]]
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[[Category: Lescar J]]
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[[Category: Lescar, J]]
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[[Category: Li Z]]
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[[Category: Li, Z]]
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[[Category: Luo D]]
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[[Category: Luo, D]]
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[[Category: Walz T]]
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[[Category: Walz, T]]
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[[Category: Wong YH]]
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[[Category: Wong, Y H]]
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[[Category: Orthogonal bundle]]
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[[Category: Rna viral nucleoprotein]]
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[[Category: Viral genomic rna encapsidation]]
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[[Category: Viral protein]]
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Current revision

Structure of the Nucleoprotein from Rift Valley Fever Virus

PDB ID 3ouo

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Proteopedia Page Contributors and Editors (what is this?)

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